Enzyme Commission Number
EC 3.4.17.2
Product Overview
Recombinant carboxypeptidase B is specially used for the hydrolysis of protein C-terminal basic amino acids (lysine, arginine, histidine), also known as peptidyl-L-lysine (L-arginine) hydrolase or protase. The isoelectric point is 5.9, and the optimum pH is 7.5-9.0. The activity is competitively inhibited by arginine and lysine, metal ion chelating agents such as EDTA inhibit enzyme activity.
Features
Ready-to-use product, accelerating research progress, enhancing application performance.
Applications
Pharmaceutical Industry
Shelf Life
Stored in lyophilized form at -18°C, it is stable for 24 months.
Color / Form
white, off-white powder
Instruction
Avoid multiple freeze-thaw cycles.
Avoid inhalation during use, prevent contact with skin or mucous membranes, and rinse with water immediately after accidental contact.
Production Methods
Genetic Engineering and Fermentation
Specific Enzyme Activity
≥170 units/mg pro
Unit Definition
25°C, pH7.6, the amount of enzyme that catalyzes the hydrolysis of 1 μmol hippuroyl-L-arginine in 1 minute is an enzyme activity unit.
Amino Acids Sequence
ASGHSYTKYNKWEIEAWIQQVATDNPDLVTQSVIGTTFEGRNMYVLKIGKTRPNKPAIFIDCGFHAREWISPAFCQWFVREAVRTYNQEIHMKQLLDELDFYVLPVVNIDGYYTWTKDRMWRKTRSTMAGSSCLGVDPNRNFNAGWCEVGASRSPCSETYCGPAPESEKETKALADFIRNNLSTIKAYLTIHSYSQMMLYPYSYDYKLPENYEELNALVKGAAKELATLHGTKYTYGPGATTIYPAAGGSDDWSYDQGIKYSFTFELRDTGFFGFLLPESQIRQTCEETMLAVKYIANYVREHLY
Shipping
Timely shipping by optional means
Specification
On customer requests
Description
Recombinant carboxypeptidase B can be used in the production of insulin and analogues, the quality detection of recombinant antibodies, the determination of protein C-terminal amino acids, small molecular protein or the production of polypeptides, such as antibacterial peptides, food flavor peptides, etc., and the enzymatic synthesis of some special compounds.
