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Bacterial 16S Sequencing

Bacterial 16S Sequencing

There are three kinds of rRNA sequences in bacteria, 23S, 16S and 5S. Among them, 16S rRNA is the most commonly used molecular clock in the systematic taxonomic study of bacteria due to its moderate nucleotide number, a large amount of information, high stability and easy extraction and analysis.16S rDNA is about 1540bp in length and exists in all bacterial chromosome genomes.

16S rRNA has a large content (about 80% of bacterial RNA content). The evolution of 16S has highly conservative in structure and function, known as bacterial fossil. In most prokaryotes, rDNA has multiple copies with the same copy number in 5S, 16S, and 23S rDNA. The 16S rRNA contains 9 variables and 10 constant regions. The conservative sequence region reflects the genetic relationship between biological species, while the highly variable sequence region can reflect the differences between species. Because of its moderate size, 16S rDNA can use sequencing technology to obtain its sequence easily, so it is accepted by bacteriologists and taxonomists.

The sequence characteristics of 16S rDNA molecules have changed the basis of molecular biology for the phylogenetic classification of related species of different taxonomic levels. At present, 16S rDNA sequence information has been widely distributed for bacterial identification and phylogenetic research.

Secondary structure model for E. coli 16S rRNA.Fig.1 Secondary structure model for E. coli 16S rRNA. (Lavender C, et al. 2015.)

Our Service Process

The service process of bacterial 16S rRNA sequencing - Creative Biogene

Service Offering

Service Package Service Content Period
Bacterial 16S rRNA identification
  • Sequencing of PCR products
  • Sequence amplification of 16S rDNA
  • Cloning and sequencing of related genes
  • Sequencing alignment analysis
1-2 weeks
Gene library construction of bacterial 16S rDNA
  • Gene library construction of bacterial 16S rDNA
  • Development of RFLP markers
  • Analysis of bacteria community diversity
5-6 weeks

Creative Biogene's bacterial 16S sequencing service provides results including,

  • Electrophoretogram of PCR products
  • Sequencing peak file and sequencing report
  • Sequence alignment and identification results
  • RFLP markers
  • Gene library of bacterial 16S rDNA.
  • Bacteria community diversity analysis

Advantages of Our Services

  • We have experienced researchers and advanced sequencing platform.
  • We will customize the experimental scheme according to your requirements, and offer you a one-stop service.
  • Our services are undertaken to GMP standards.
  • We have a well-established quality management system and have passed ISO 9001:2015 certification.
  • We regularly accept audits from our customers to ensure that we meet their high standards.

Creative Biogene is an expert in microbial identification services and has accumulated years of experience in bacterial 16S rRNA sequencing. Our advanced sequencing platform ensures that we provide you with efficient standard service procedures, high-quality experimental data and comprehensive bioinformatics analysis. Our services including DNA extraction of bacteria, PCR amplification, purification, sequencing, and analysis.

If you are interested in our services, please contact us for more details.

Reference

  1. Lavender C, et al. (2015). “Model-free RNA sequence and structure alignment informed by SHAPE probing reveals a conserved alternate secondary structure for 16S rRNA.” Plos Computational Biology. 11(5): e1004126.
For Research Use Only.

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