The cells of Pedomicrobium are spherical, oval, rod-shaped, pear-shaped or bean-shaped, 0.4-2.0μm wide, and unevenly colored. The bacteria reproduce primarily by budding at the tips of cell extensions (hyphae) of uniform diameter (0.15-0.3 microns). Daughter cells may remain attached to their parent cell hyphae or they may leave as monoflagellated "swimmers". After growing in size, mature motile cells grow one or more hyphae from several locations on their cell surface. In addition, the division of a single parent cell simultaneously with the budding process can be seen occasionally.
Figure 1. Electron micrograph of Pedomicrobium. (Gebers, R., et al.; 1988)
Pedomicrobium is a Gram-negative, micro-aerobic to aerobic bacterium. Precipitation of ferric and/or manganese sometimes occurs on mother cells or on hyphae. Pedomicrobium is a chemoheterotroph. It grows on inorganic salt media containing simple organic compounds or organic and inorganic complexes containing fulenoic acids and chaperone oxides. Precipitation of iron, manganese and mucus was mainly on the mother cells.
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The parent cell of Pedomicrobiun ferrugineum is spherical, oval or rod-shaped, 0.6-2.0μm in diameter, with 1-4 roots (or more). Wherein, the width of the mycelium is 0.2 microns or less. Cells stained unevenly. Individual buds at the tip of the hyphae are initially spherical to ovoid and darker stained. Later the buds can move by unipolar flagella. In addition, interstitial buds have also been observed.
Surface colonies of Pedomicrobiun ferrugineum are brownish and reach 0.5 mm in diameter after a few months. Only when the medium contains organic-inorganic complexes of fulvenoic acid and chaperone oxides, it develops more deeply in agar. Iron hydroxide precipitation occurs mainly on mother cells and only later on some hyphae. The bacteria do not oxidize or precipitate manganese.
Pedomicrobiun ferrugineum was originally isolated from the soil of the Leningrad region, which contains iron and manganese deposits. The bacteria are widely distributed.
Pedomicrobium manganicum mother cells are spherical to ovate, 0.4μm in diameter, with 1 to 4 or more hyphae and a single round to oval bud at the tip of the hyphae. The hyphae have a width of 0.2 microns or less. The hyphae tend to be bifurcated and vary in length, with interstitial buds. Colonies were small and black on agar culture medium containing organic-inorganic complexes of fulveenoic acid and accompanying oxides. Manganese was mainly deposited on the mother cells, and no iron precipitation was observed. Pedomicrobium manganicum is chemoheterotrophic and microaerobic.
Pedomicrobium manganicum was originally isolated from soils in the Leningrad region containing manganese deposits and is widely distributed.
The mother cell of Pedomicrobium podsolicum is spherical, and a few parts are oval. Egg cells are 0.6-1.0 microns wide, with 1 to 4 hyphae and a single spherical bud at the tip of the hyphae. Direct germination of mother cells has been observed. The hyphae were 0.2 μm or less in width, very short and often bifurcated. The colonies grown on the medium containing the organic-inorganic complex of humus were small and brownish-black. Ferric hydroxide and manganese hydroxide precipitate on the mother cells. Originally isolated from calcified soil.
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The culture of Pedomicrobium requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.