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Vitreoscilla Cultivation

Vitreoscilla Cultivation

The protonema of Vitreoscilla are colorless and 1.2-2x3-70 microns in size. The colony edges of Vitreoscilla consist of distinct cylindrical or barrel cells. The cells of Vitreoscilla reproduce by breaking and moving in a sliding fashion. Gram staining of the bacteria was negative.

Vitreoscilla's organic energy nutrition is presumed to be only respiratory metabolism. Previous studies have found that the bacteria cannot hydrolyze proteins. This bacterium has only been reported to grow on complex media and its minimum nutritional requirements are not known. In addition, Vitreoscilla does not form intracellular sulfur particles from hydrogen sulfide.

Vitreoscilla Culture Service

Bacteria exist widely in nature, but most of them are mixed together, which is very difficult to study in isolation. Therefore, bacterial researchers need to isolate and explore suitable culture conditions before conducting individual bacterial species research. But this process is complicated and time-consuming. Creative Biogene provides customizable Vitreoscilla strain culture services. This service can help you obtain cultures of Vitreoscilla for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Vitreoscilla beggiatoides

In the protonema, the cells of Vitreoscilla beggiatoides are cylindrical with blunt ends, and can move in a sliding manner at a slow speed (approximately 2 μm/s).

Vitreoscilla moniliformis

Protonema of Vitreoscilla moniliformis in chains. The size of protonema is 2.2-3.0x30-150 μm. Each protonema may be sausage-shaped, up to 3.0 μm in diameter. The bacteria can move in a sliding manner, and can move in a sliding manner at a slow speed (approximately 2 microns/minute).

Vitreoscilla stercoraria

Scanning electron micrographs depicting the differences between the barrel-shaped cells of V. stercoruriu VT1 arranged in chains and the continuous outer cell wall exemplified by strain B23SS.Figure 1. Scanning electron micrographs depicting the differences between the barrel-shaped cells of V. stercoruriu VT1 arranged in chains and the continuous outer cell wall exemplified by strain B23SS. (W. Strohl, et al.; 1986)

The protonema of Vitreoscilla stercoraria are 1.2-1.5 x 100 μm in size and often have irregular inflections. The cells within the protonema are sausage-shaped and are 1.2-1.5x1.2-12 microns in size. Sliding motion can be performed, and the motion is slower, about 2 microns per minute.

Vitreoscilla filiformis 

When Vitreoscilla filiformis was on agar media, its colonies were arc-shaped or annular like some strains of oscillloria.

Vitreoscilla catenula 

The protonema of Vitreoscilla catenula are cylindrical and 1.5-2.0 microns wide. The barrel cells involved in the formation of protonema are 3-6 μm long and have constricted parts between cells.

Why Choose Us?

The culture of Vitreoscilla requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.

References

  1. Bergey's Manual of Systematic Bacteriology Book Review Int. J. of Syst. Bact. 1985, p. 137.
  2. W. Strohl, et al.; Characterization of Vitreoscilla beggiatoides and Vitreoscilla filiformis sp. nov., nom. rev., and comparison with Vitreoscilla stercoraria and Beggiatoa alba. International Journal of Systematic and Evolutionary Microbiology. 1986, 36 (1986): 302-313.
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