Lactobacillus salivarius Cultivation

Probiotics are the normal flora in the intestinal tract of humans and animals, and can be used to develop functional foods. Functional foods are an emerging field that promotes health and provides consumers with a variety of food choices. As an important probiotic in animals, Lactobacillus can regulate the balance of intestinal flora and enhance the body's immunity and resistance. Among the Lactobacillus species, Lactobacillus salivarius is the most common bacteria in human saliva. Lactobacillus salivarius is a gram-positive bacillus, without catalase and oxidase, and can produce lactic acid. Lactobacillus salivarius has a great potential to adhere to the pharyngeal mucosa, and has the effect of resisting some intestinal or vaginal lactobacilli and eradicating Streptococcus agalactiae (GBS), which can be used in new milk-based probiotic fermented foods. Other Lactobacillus salivarius strains have been shown to be well tolerated and safe in animal models and human clinical trials, including in pregnant women. Lactobacillus salivarius, as a probiotic Lactobacillus with great potential, has become a research hotspot in recent years, and is being widely used to make probiotic preparations suitable for humans and animals.

Biological Characteristics of Lactobacillus salivarius

Lactobacillus salivarius belongs to the Lactobacillus family and the genus Lactobacillus. The size is generally 0. 6-9μm ×1.5-5μm, rounded at both ends, single, twin, and chains of variable length, immotile, aflagellate, gram-positive, non-spore-forming rod-shaped bacteria. The cell wall contains glyceroteichoic acid, and the peptidoglycan is L-lysine-D-aspartic acid type; the nutritional requirements are complex, requiring the addition of calcium pantothenate, riboflavin and folic acid; no growth at 15 ℃, variable growth at 45 ℃, The optimum temperature is 35 to 40 ℃. Lactobacillus salivarius can metabolize fructooligosaccharides to produce lactic acid and acetic acid, which can be cultured in vitro on MRS medium, and the optimal culture temperature is 37 °C. In addition, Lactobacillus salivarius has good acid and bile salt resistance properties and can survive in the stomach and intestines of humans and animals. Lactobacillus salivarius colonies Lactobacillus salivarius can be cultured on MRS medium. After streaking on this medium, it grows well, and its colonies are usually rough, 1 to 3 mm in diameter, white to light gray, with neat edges, shiny and raised on the surface of the medium. After 24 hours of cultured strains were stained with gram, the strains were blue straight rods, arranged in single, double or aggregated form, and the two ends were blunt, and the isolated strains were gram-positive bacilli. 

Lactobacillus salivarius Culture Service

Figure 1. Scanning electron micrograph of the probiotic strain Lactobacillus salivarius.Figure 1. Scanning electron micrograph of the probiotic strain Lactobacillus salivarius. (Roy D Sleator.,2010)

Creative Biogene offers customizable Lactobacillus salivarius strain culture services. This service can help you obtain Lactobacillus salivarius cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Microbial GMP Production

Creative Biogene's fermentation platform has Good Manufacturing Practices (GMP) and can provide customers with a wide range of high-quality microbial fermentation products such as active pharmaceutical ingredients, enzymes and various fine chemicals. In addition, our microbiology experts have completed the transformation and innovation of traditional processes through continuous breakthroughs in key technologies of microbial fermentation processes, and fully contributed to the smooth delivery of the project.

Production Capacity

Creative Biogene builds a world-class microbial fermentation technology platform, providing a variety of services from strain screening and optimization to fermentation production and product purification. We have many years of rich experience and provide good technical support for microbial GMP production.

Facility Display

As a leader in microbial production, Creative Biogene has comprehensive production process technology and high-volume manufacturing capabilities. Our goal is to help our customers develop streamlined and controlled manufacturing processes and to support customers throughout the entire product development process, from the R&D stage to market launch.

Device Example:

  • Fermentation, centrifugation and filtration upstream process equipment;
  • Fully automatic fermenters ranging in volume from 4,000L to 12,000L with a total capacity of over 100,000 liters;
  • From industrial-scale chromatography systems, membrane systems to larger-scale continuous centrifuges;
  • Recycling and Downstream Equipment;
  • Waste treatment equipment.

Why Choose Us?

The culture of Lactobacillus salivarius requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.


  1. Raftis E J, et al.; Genomic Diversity of Lactobacillus salivarius. Applied and Environmental Microbiology. 2011, 77(3):954-965.
  2. Perez-cano F J,et al.; In vitro immunomodula- tory activity of Lactobacillus fermentum CECT5716 and Lactoba- cillus salivarius CECT5713: two probiotic strains isolated from human breast milk. Immunobiology. 2010, 215 (12):996- 1004.
  3. Langa S, et al.; Characterization of Lacto- bacillus salivarius CECT 5713,a strain isolated from human milk:from genotype to phenotype. Applied Microbiology and Biotechnology. 2012, 94(5):1279-1287.
  4. Arribas B, et al.; The immunomodu- latory properties of viable Lactobacillus salivarius ssp. salivarius CECT5713 are not restricted to the large intestine. European Journal of Clinical Nutrition. 2012, 51(3):365-374.
  5. Roy D Sleator. Probiotic therapy-recruiting old friends to fight new foes. Gut Pathogens. 2010, 2(1), 5.
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