Thiopedia of individual bacteria are spherical to oval and reproduce by binary division. Bacterial cells are arranged in plates with typical quadruplexes as constituent units. Bacterial cells undergo successive divisions in two vertical planes to form small plate colonies of 4, 8, 16, 32 and 64 cells. The study observed that the bacterial cells were unable to move.
Thiopedia is a gram-negative bacteria. Bacterial cells contain bacteriochlorophyll a and carotenoids, two pigments located on the inner membrane of the sac or pile. There are air bubbles in the central part of the bacterial cell. Thiopedia belongs to anaerobic bacteria according to whether the bacteria are dependent on oxygen or not. In the presence of hydrogen sulfide, bacteria can perform photosynthesis. Among them, spherical elemental sulfur, which is produced and stored as an intermediate oxidation product, is located in the bubble-free part of the cell. During photosynthesis, molecular hydrogen acts as an electron donor, but does not release molecular oxygen. Due to the photosynthetic chromophores, the cell suspensions have different colors from pink to purplish red.
The cells of Thiopedia rosea are slightly longer spherical or oval, 1-2×1.2-2.5 microns in size. Bacteria are regularly arranged in plates. Individual cells are gray, and cell suspensions are pink to red-violet in color.
Figure 1. Photosynthetic Purple Sulphur Bacteria: top, Thiopedia sp; bottom, Chromatium sp.()
Thiopedia rosea is an obligate photoautotroph and is strictly anaerobic. The optimum pH range for growth is: 7.2-9.0. Optimum growth temperature: 20°-25°C. In photosynthesis, the photosynthetic electron donor is sulfide or sulfur. And the bacteria are very sensitive to hydrogen sulfide. Under natural conditions, Thiopedia rosea is widely distributed in illuminated hydrogen sulfide-containing mud and in fresh or salty stagnant water or hot springs.
The culture of Thiopedia requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.