Genome editing is a new and accurate genetic engineering technology that can modify specific target genes in the genome of organisms. Through genomic research on microbes, new special enzyme genes, functional genes related to important metabolic processes as well as metabolite production are continuously discovered. The technology has application value in the biopharmaceutical, pollution control, energy and chemical bio-manufacturing industries, and has shown great potential in genetic research, gene therapy and genetic improvement.
Early microbial genome editing mainly focused on random screening and simple rational screening, but the shortcomings of traditional methods that are time-consuming, labor-intensive, work-intensive, and non-directional mutagenesis results have gradually been exposed over time. With the development of modern molecular biology technology, there are many new methods of microbial breeding with more directional and positive mutation, which greatly promotes the accuracy and efficiency of microbial genome editing. Creative Biogene has launched a microbial genome editing service based on the Rec homologous recombination method and the CRISPR/Cas9 platform.
Microbial genome editing based on homologous recombination uses its own Rec system to carry out homologous recombination of exogenous DNA to achieve allelic replacement of target genes, and recombination of genetic information between two homologous strands of DNA. By producing and isolating DNA fragments with genomic sequences similar to the part of the genome to be edited, these fragments are injected into monocytes. Once these fragments enter the cell, they can be recombined with the cell's DNA to replace the target part of the genome.
Microbes in nature always use various protection mechanisms that enable them to coexist with harsh environments and invasive nucleic acids. Many microbes can resist nucleic acid invasion through specific methods based on gene sequence. CRISPR/Cas9 is one of these protection mechanisms and the most widely used genome editing technology. We provide the CRISPR/Cas9 method for microbial genome editing services (including gene knockout, site-directed mutation, site-directed insertion of foreign sequences, etc.). Compared with traditional gene knockout methods, this method is more convenient for subsequent experimental research.
Fig.1 Mechanism of CRISPR/Cas9 action. Arora L et al. 2017)
Creative Biogene's microbial genome editing service including the following items,
Creative Biogene is a forward-looking research institution and a leading customer service provider in the field of microbial genome editing. Creative Biogene is committed to providing you with the best genome editing services including knockout, knock-in and mutations. The available species are Escherichia Coli, Bacillus Subtilis, Salmonella, and Lactobacillus, etc. The whole process is focused on making an informed decision so that you can move forward with confidence.
If you are interested in our services, please contact us for more details.