Lactobacillus fermentum Cultivation

Lactobacilli are the normal flora of the oral cavity and are Gram-positive facultative anaerobic or obligate anaerobic bacilli. Lactobacillus can be divided into two categories: one is homologous fermentation strains, which mainly produce lactic acid after fermentation using glucose. This group is represented by Lactobacillus casei and Lactobacillus acidophilus, which are closely related to caries. The other type is heterologous fermentation strains, which produce lactic acid and a larger amount of acetic acid, ethanol and CO2 after fermentation, which is represented by Lactobacillus fermentum. The most commonly isolated species in saliva samples was Lactobacillus acidophilus, and the most common in dental plaque was Lactobacillus fermentum. Lactobacillus fermentum, named after it causes fermentation, is an obligate heterotypic fermenting strain. It can be used as a probiotic culture. Lactobacillus fermentum is one of the most abundant species found in natural whey cultures. It does not grow at 15°C, but does grow at 45°C. Single or double pound fermentation bars vary widely in length. Lactobacillus fermentum can use arabinose, cellobiose, galactose, maltose, mannose, melibiose, raffinose, ribose, sucrose, trehalose and xylose) for fermentation. Lactobacillus fermentum has 52-54% G+C content (mol%) and d-aspartyl-l-ornithine-type peptidoglycan. In addition, the bacteria also exhibited catalase activity.

Lactobacillus fermentum Culture Service

Figure 1. Gram staining microscopic examination of Lactobacillus fermentum.Figure 1. Gram staining microscopic examination of Lactobacillus fermentum.(Liu J, et al.; 2021)

Creative Biogene offers customizable Lactobacillus fermentum strain culture services. This service can help you obtain Lactobacillus fermentum cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Microbial GMP Production

Creative Biogene's fermentation platform has Good Manufacturing Practices (GMP) and can provide customers with a wide range of high-quality microbial fermentation products such as active pharmaceutical ingredients, enzymes and various fine chemicals. In addition, our microbiology experts have completed the transformation and innovation of traditional processes through continuous breakthroughs in key technologies of microbial fermentation processes, and fully contributed to the smooth delivery of the project.

Production Capacity

Creative Biogene builds a world-class microbial fermentation technology platform, providing a variety of services from strain screening and optimization to fermentation production and product purification. We have many years of rich experience and provide good technical support for microbial GMP production.

Facility Display

As a leader in microbial production, Creative Biogene has comprehensive production process technology and high-volume manufacturing capabilities. Our goal is to help our customers develop streamlined and controlled manufacturing processes and to support customers throughout the entire product development process, from the R&D stage to market launch.

Device Example:

  • Fermentation, centrifugation and filtration upstream process equipment;
  • Fully automatic fermenters ranging in volume from 4,000L to 12,000L with a total capacity of over 100,000 liters;
  • From industrial-scale chromatography systems, membrane systems to larger-scale continuous centrifuges;
  • Recycling and Downstream Equipment;
  • Waste treatment equipment.

Why Choose Us?

The culture of Lactobacillus fermentum requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.


  1. Salam A.Ibrahim. Lactic Acid Bacteria: Lactobacillus spp.: Other Species Encyclopedia of Dairy Sciences. 2011, Pages 125-131.
  2. Naghmouchi K, et al.; Lactobacillus fermentum: a bacterial species with potential for food preservation and biomedical applications. Crit Rev Food Sci Nutr. 2020, 60(20):3387-3399.
  3. Liu J, et al.; Lactobacillus fermentum ZS09 Mediates Epithelial-Mesenchymal Transition (EMT) by Regulating the Transcriptional Activity of the Wnt/β-Catenin Signalling Pathway to Inhibit Colon Cancer Activity. J Inflamm Res. 2021, 14:7281-7293.
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