Pichia pastoris is widely used to produce a variety of exogenous recombinant proteins, and as a model organism to study peroxisome biogenesis as well as methanol assimilation. However, it also has some disadvantages, especially for gene modification, the efficiency of homologous recombination is low. Since targeted gene editing requires several hundred or more homologous overhanging base pairs, the editing efficiency is only 0.1% ~ 30%. Therefore, implementing a highly efficient and convenient gene editing tool is urgently needed.
Creative Biogene has developed and expanded the homologous recombination and CRISPR/Cas9 systems for Pichia pastoris to achieve single-gene editing, multi-gene editing and multi-copy gene editing with high efficiency.
Over 5000 different proteins have been successfully produced through expression platform of Pichia pastoris because of its rapid growth rate, high cell density cultivation, good post-translational modification without excessive glycosylation and strong inducible promoter. Through Pichia pastoris genome editing, its protein production platform can be better used to produce more proteins for use in biopharmaceuticals and other fields.
Creative Biogene uses two methods to achieve Pichia pastoris genome editing, they are,
The specific content of the Pichia pastoris genome editing service provided by Creative Biogene includes,
In general, our turnaround time is 4-8 weeks depending on the size of your project.
Creative Biogene has excellent microbiology experts, well-established gene editing platforms, and professional bioinformatics analysis to provide you with a one-stop service in Pichia pastoris genome editing. We believe that our services will make your project more effective.
If you are interested in our services, please contact us for more details.