consultation 

Clonothrix Cultivation

Clonothrix Cultivation

The mycelium of Clonothrix is up to 1.5cm long, attached or detached. The mycelium is wrapped in a more or less pronounced sheath. This sheath is embedded with iron or manganese compounds, so it has a yellowish-brown color. The mycelia are tapered, they may be solitary, or with pseudo-branching.

Figure 1. Transmission electron microscopy of C. fusca filaments.Figure 1. Transmission electron microscopy of C. fusca filaments. (Vigliotta G, et al.; 2007)

Cells of Clonothrix are colorless or bluish cylindrical; cell clumps may appear brown. The bacteria can be isolated as individual cells and then propagated by breaking the sheath and releasing the cells outside. Such cells often attach themselves in parallel to old cells and produce new mycelium, resulting in "pseudobranching".

Clonothrix Culture Service

Creative Biogene offers customizable Clonothrix strain culture services. This service can help you obtain Clonothrix cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Clonothrix fusca

The sheathed mycelium of Clonothrix fusca tapers towards the apex. One or more of its mycelia can be attached to the same substrate. Older parts of the mycelium appear light tan (7 μm in diameter), while younger parts are pale blue (3 μm in diameter). The bacteria reproduce in pseudo-branches, and the pseudo-branches are interdigitated.

Clonothrix fusca cells are 2-2.5x12-18 microns in size, with larger cells at the base of the mycelium and smaller at the tip. Rarely, mycelial cells can bud from near their ends to produce distinct multicellular ampoules. These processes can serve as a means of reproduction, but the mechanism is unknown. They were initially found attached to iron fittings in well water containing Aplococcus natans at 14°C.

Clonothrix gracillima

The mycelium of Clonothrix gracillima is slender, never tapering to the apex. The sheath is narrow, hard and colorless. Cells of Clonothrix gracillima are cylindrical, 1.5x4.5-6.0 μm in size, with a light bright blue color. False branches of Clonothrix gracillima are long, pliable, intertwined with interdigitated secondary false branches. It was originally found and isolated from a manger.

Why Choose Us?

The culture of Clonothrix requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.

References

  1. Bergey's Manual of Systematic Bacteriology Book Review Int. J. of Syst. Bact.1985, p. 167.
  2. Vigliotta G, et al.; Clonothrix fusca Roze 1896, a filamentous, sheathed, methanotrophic gamma-proteobacterium. Appl Environ Microbiol. 2007 Jun;73(11):3556-65.
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