Alysiella cells are 0.6x2.3μm in size and pair side by side and form filaments. The filaments are flat rather than cylindrical, and the terminal cells are not round. Sliding motion is performed when the flat surface of the filament faces the substrate. The bacteria have no spores and no capsule. Gram negative. Its organic energy nutrition is fermentative metabolism. Growth is best in the presence of serum or blood, and minimum nutritional requirements are not known.
Alysiella is a strictly aerobic bacteria. The optimum growth temperature is 37°C. The natural habitat is the oral cavity of animals.
Creative Biogene offers customizable Alysiella strain culture services. This service can help you obtain Alysiella cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.
Alysiella filiformis cells are 0.6x2-3 μm in size, arranged in pairs side by side to form flattened filaments that are 0.5-1 μm thick and vary in length. Terminal cells do not differ morphologically from cells within the filament. The bacteria can slide on solid substrates. Gram negative. It grows best in the presence of blood or serum. After 3-4 days of culture, the agar-agar colonies are low in convexity and 1-1.5 μm in diameter. Colonies are smooth, translucent wavy and often have a "fringe" of narrow sliding filaments. The colony has no pigment and odor. Shows β-hemolysis in blood agar medium.
Figure 1. Alysiella filiformis. (From Bacdive)
Granular and cloudy on serum broth medium, and a moderate amount of pellet formed. Does not grow on Simmon's citrate medium. In agar or shallow liquid medium, the bacteria can use fructose, glucose, maltose, sucrose, trehalose to produce acid but not gas, while using inulin and ribose may or may not produce acid. The bacteria cannot utilize arabinose, cellobiose, dulcitol, erythritol, galactose, glycerol, inositol, lactose, mannitol, mannose, melanose, melabiose, raffinose, rhamnose, salicylic alcohol, sorbitol, sorbitol or xylose to produce acid.
Alysiella filiformis sometimes weakly liquefies gelatin, does not liquefy coagulated serum, does not hydrolyze casein, and does not peptonize litmus milk. During the culture, the bacteria can produce H2S, negative for urease, do not produce indole, and do not hydrolyze starch, sodium hippurate and esculin. Neither nitrate nor methylene blue could be reduced by Alysiella filiformis. Methyl red and V.P. assays were negative. Oxidase positive, contact enzyme indeterminate. The natural habitat is the oral cavity of the animal.
The culture of Alysiella requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.