Activated Sludge Microecology

Activated Sludge Microecology

Activated sludge is a general term for microbial communities and the organic and inorganic substances to which they are attached. It is mainly used for wastewater treatment. The activated sludge method is a kind of aerobic treatment method that uses suspended microbial flocs to treat organic sewage. The complex microbes in the activated sludge and the organic nutrients in the wastewater form a complex food chain, in which microbes are mainly composed of bacteria. The bacteria mainly include micellar bacteria and filamentous bacteria, they play the most important role in the treatment of organic wastewater. In addition, there are micro-animals such as protozoa and metazoa in the activated sludge, and they are called indicator organisms in the activated sludge system. The quality of the treated water is related to the type, quantity and activity of the microbes which constitute the activated sludge.

Technical Process

  • The process of activated sludge microbe high-throughput sequencing analysis.

The process of activated sludge microbe high-throughput sequencing analysis - Creative Biogene

  • The process of activated sludge microbe PCR-DEEG analysis.

The process of activated sludge microbe PCR-DEEG analysis - Creative Biogene

Sample Type and Detection Type

  • The sample type is the original sample of sewage.
  • The requirements of DNA sample: total DNA ≥ 300ng, DNA concentration ≥ 10ng/μL, OD260/280 = 1.8 - 2.0.
  • The detection types are bacteria, fungi, viruses, etc.

What Can We Offer?

Creative Biogene uses high-throughput sequencing, PCR-DGGE, real-time fluorescent quantitative PCR and other methods to sequence DNA in samples. Through statistical and bioinformatics analysis, a large amount of data is processed to reveal the types of microbes in activated sludge and their relative abundance, explore microbial diversity, and study the correlation between activated sludge microbes and the environment.

The specific service content includes the following aspects, microbial isolation & cultivation, microbial identification, microbial sequencing, microbial proteomics, microbial metabonomics and bioinformatics analysis.

Bioinformatics analysis

OTU clustering Taxonomic analysis Phylogenetic analysis Analysis based on OUT Gene function  analysis
OTU distribution
Rarefaction curve
Shannon index curve
Rank abundance curve
Diversity index
Community composition analysis
Community correlation analysis
Community difference analysis
  • NMDS analysis
  • PCA
  • PCoA
  • LEfSe differential analysis
  • Network diagram
UniFrac analysis
Phylogenetic tree
OTU correlation analysis
  • Heatmap
  • Venn diagram
KEGG analysis
GO analysis
MetaCyc analysis
EggNOG analysis
CAZy analysis
CARD analysis

Our Advantages

  • We have a mature bioinformatics analysis platform and professional scientists.
  • We can customize the solution and combine it with a quick turnaround at an affordable cost.
  • We will feedback progress to customers in time to ensure that your requirements are met.

Activated sludge is indispensable in domestic and industrial sewage treatment. Creative Biogene is committed to providing the best service to accelerate the realization of customers' research goals. Our activated sludge microecology solution is excellent in speed, accuracy and sensitivity, the result we offer is with good reproducibility. We look forward to working with you for your cooperation.

If you are interested in our services, please contact us for more details.


  1. Zhang L, et al. (2019). "Effect of continuous and intermittent electric current on lignin wastewater treatment and microbial community structure in electro-microbial system." Scientific report. s 9.1: 1-11.
For Research Use Only.
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