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Sporocytophaga Cultivation

Sporocytophaga Cultivation

The shape of Sporocytophaga cells are flexible single rods with rounded ends. Cells are 0.3-0.5x5-8 microns in size. In old cultures, globoids and malformed cells appeared, and microsporocysts were formed. The cells of Sporocytophaga move in a sliding fashion. The bacteria were negative by Gram staining.

Phase-contrast micrograph of fiber debris containing vegetative cells and microcysts of Sporocytophaga in enrichment culture on cellulose.Figure 1. Phase-contrast micrograph of fiber debris containing vegetative cells and microcysts of Sporocytophaga in enrichment culture on cellulose. (B. Hofsten, et al.; 2004)

Sporocytophaga carries out organic energy nutrition through respiratory metabolism. In this process, molecular oxygen is used as the final electron acceptor. Of these, cellobiose, cellulose, glucose and mannose (for some strains) are the only known carbon and energy sources. Ammonium, nitrate ions, peptone, urea or yeast extract can be the sole nitrogen source. Amino acids, peptone, yeast extract or nutritional agar cannot be the only source of carbon and energy. During the culturing process, there is no need to add organic growth factors to the medium.

Sporocytophaga are strictly aerobic bacteria. The optimum temperature is around 30°C.

Sporocytophaga Culture Service

Bacteria exist widely in nature, but most of them are mixed together, which is very difficult to study in isolation. Therefore, bacterial researchers need to isolate and explore suitable culture conditions before conducting individual bacterial species research. But this process is complicated and time-consuming. Creative Biogene provides customizable Sporocytophaga strain culture services. This service can help you obtain cultures of Sporocytophaga for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Sporocytophaga myxococcoides

The cells of Sporocytophaga myxococcoides are flexible single rods with rounded ends. Cells are 0.3-0.5x5-8 microns in size. Circular bodies or abnormally long cells may appear in old cultures. Microsporangia in the dormant stage are round and about 1.5 microns in diameter. When grown on cellulose medium, growing (vegetative) cells and microcysts were smaller than those on glucose medium. Electron microscopy studies showed that the vegetative cells had the fine structure typical of Gram-negative bacteria, while the microsporangia had a thick ciliated outer capsule encased in a highly coiled cell wall. The growth was viscous on cellulose (filter paper) agar (or silica gel) or glucosamine. And due to the presence of extracellular mucus, the liquid medium becomes viscous. The filter paper on the surface of agar or silica is finally digested, so a translucent area appears around the colony. Colonies protrude on glucosamine agar medium. Culture conditions and genus characteristics are the same.

Why Choose Us?

The culture of Sporocytophaga requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.

References

  1. Bergey's Manual of Systematic Bacteriology Book Review Int. J. of Syst. Bact. 1985, p. 133.
  2. B. Hofsten, et al.; Observations on bacteria occurring together with Sporocytophaga in aerobic enrichment cultures on cellulose. Archiv für Mikrobiologie. 2004, 79: 69-79.
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