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SILAC Quantitative Proteomics

SILAC Quantitative Proteomics

SILAC refers to stable isotope labeling with amino acids in cell culture. This method was first developed in 2002 by the laboratory of Mann, Denmark, who made further improvements to the AACT technology. They applied this method to quantitative proteome research for the first time, providing an effective scheme for comprehensive and systematic qualitative and quantitative analysis of complex cell proteome.

SILAC technology uses medium containing light, medium or heavy isotope-labeled essential amino acids (mainly Lys and Arg) to label newly synthesized proteins with stable isotopes during cell growth. Mix all types of proteins in equal amounts, and perform mass spectrometry analysis after enzymolysis. Relative quantification is carried out by comparing the area size of unlabeled/isotope-labeled mass spectra in the primary mass spectrum, and the peptides are sequenced in the secondary mass spectrometry for protein identification.

Flowchart of triple SILAC coupled with LC-MS/MS for the comparative analysis of three distinct cell populationsFig.1 Flowchart of triple SILAC coupled with LC-MS/MS for the comparative analysis of three distinct cell populations. (Hara Y, et al. 2013.)

Application of SILAC Quantitative Proteomics

SILAC is widely used in comparative proteomics, protein-protein interaction, protein-DNA interaction and protein-RNA interaction, etc. SILAC can be applied to disease marker screening, mechanism research, drug target research and special functional protein screening in the field of medicine and health.

Application of SILAC quantitative proteomics - Creative Biogene

Our Service Process

The service process of SILAC quantitative proteomics - Creative Biogene

Service Offering

Creative Biogene’s SILAC quantitative proteomics service provides results including,

  • Raw data
  • Experimental steps
  • Related mass spectrometry parameters
  • Protein identifications and intensities 
  • Mass spectrum picture
  • Bioinformatics analysis report

Our bioinformatics analysis covers a wide range including data quality control, functional annotation and enrichment analysis, clustering analysis, network analysis and statistical analysis, etc.

Sample Type

Cells, tissues, urine, whole blood, plasma, total protein, etc.

Turnaround Time

In general, our turnaround time is 2-5 weeks depending on the size of your project.

Advantages of Our Service 

  • High-throughput, multiple samples are mixed, separated, digested and identified simultaneously, reducing the quantitative error.
  • In vivo marker, the result is closer to the real physiological state.
  • Isotope labeling has high efficiency and stability, and is not affected by the lysate. The result is reproducible and reliable.
  • High sensitivity and small sample size requirements.

Creative Biogene has excellent microbiology experts, well-established proteome technology platforms, and professional bioinformatics analysis to provide you with a one-stop service in SILAC quantitative proteomics. Our services include cell culture, cell labeling, protein extraction, protease digestion, peptide separation, mass spectrometry and bioinformatics analysis.

If you are interested in our services, please contact us for more details.

Reference

  1. Hara Y, et al. (2013). "Quantitative proteomic analysis of cultured skin fibroblast cells derived from patients with triglyceride deposit cardiomyovasculopathy." Orphanet journal of rare diseases. 8.1: 197.
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