SILAC refers to stable isotope labeling with amino acids in cell culture. This method was first developed in 2002 by the laboratory of Mann, Denmark, who made further improvements to the AACT technology. They applied this method to quantitative proteome research for the first time, providing an effective scheme for comprehensive and systematic qualitative and quantitative analysis of complex cell proteome.
SILAC technology uses medium containing light, medium or heavy isotope-labeled essential amino acids (mainly Lys and Arg) to label newly synthesized proteins with stable isotopes during cell growth. Mix all types of proteins in equal amounts, and perform mass spectrometry analysis after enzymolysis. Relative quantification is carried out by comparing the area size of unlabeled/isotope-labeled mass spectra in the primary mass spectrum, and the peptides are sequenced in the secondary mass spectrometry for protein identification.
Fig.1 Flowchart of triple SILAC coupled with LC-MS/MS for the comparative analysis of three distinct cell populations. (Hara Y, et al. 2013.)
SILAC is widely used in comparative proteomics, protein-protein interaction, protein-DNA interaction and protein-RNA interaction, etc. SILAC can be applied to disease marker screening, mechanism research, drug target research and special functional protein screening in the field of medicine and health.
Creative Biogene’s SILAC quantitative proteomics service provides results including,
Our bioinformatics analysis covers a wide range including data quality control, functional annotation and enrichment analysis, clustering analysis, network analysis and statistical analysis, etc.
Cells, tissues, urine, whole blood, plasma, total protein, etc.
In general, our turnaround time is 2-5 weeks depending on the size of your project.
Creative Biogene has excellent microbiology experts, well-established proteome technology platforms, and professional bioinformatics analysis to provide you with a one-stop service in SILAC quantitative proteomics. Our services include cell culture, cell labeling, protein extraction, protease digestion, peptide separation, mass spectrometry and bioinformatics analysis.
If you are interested in our services, please contact us for more details.