Viral RNA Sequencing

New coronavirus (COVID-19)

RNA sequencing is a transcriptome analysis technique using high-throughput sequencing. It can provide an accurate estimate of isoform abundance over a wide dynamic range. The wide dynamic range enables us to determine the high and low expression levels in single viral RNA sequencing experiments, thus greatly improving the efficiency of analysis. Therefore, RNA-seq is significant in both basic research and clinical practice. Viral RNA sequencing refers to the sequencing of all the viral transcriptional information, which is time-saving and economical. In addition, using long-read sequencing (LRS) to solve complex transcriptome structures has become a popular research approach.

Application of Viral RNA Sequencing

With the global spread of the new coronavirus (COVID-19) and the continuous development of sequencing technology, next-generation sequencing technology has been used in clinical and environmental sample analysis to identify possible viral pathogens. Viral RNA sequencing has discovered numerous new, uncharacterized viruses in many virus families. Besides, comparing the sequences of archived viruses collected over the years allows us to study the evolution of viruses and help design new vaccines.

Application of viral RNA sequencing- Creative Biogene

Our Service Process

The service process of viral RNA sequencing - Creative Biogene

Sample Requirements

Sample Type Library Type Amount Purity
Total RNA Viral RNA library ≥50ng OD260/280 = 1.8-2.2
OD260/230 ≥ 1.8

Service Offering

Each viral RNA sequencing project includes,

  • Collect and process samples, then extract the RNA of the sample.
  • Preliminary quality control inspection on all samples, and the QC report returned to review.
  • Using our optimized approach to prepare the library.
  • RNA sequencing at a depth and a length depended on your research purpose.
  • Bioinformatics analysis report.

Our bioinformatics analysis covers a wide range including genome/transcriptome assembly, functional profiling and other advanced analysis, etc.

Turnaround Time

In general, our turnaround time is 1-2 weeks depending on the size of your project.

Advantages of Our Services 

  • We have professional experts and we are in a leading position in microbial research.
  • We constantly strive for excellence in quality to provide first-class services.
  • Our team is very experienced and has completed numerous microbial viral RNA sequencing projects.
  • Our advanced sequencing platform, allowing us to provide high-quality data and affordable price.
  • We can combine the demands of customers, negotiate to determine the content of services

Creative Biogene has excellent microbiology experts, well-established high-throughput sequencing platforms, and professional bioinformatics analysis to provide you with a one-stop service in viral RNA sequencing. Our services include sample collection, quality control, library construction, sequencing and analysis.

If you are interested in our services, please contact us for more details.

Reference

  1. Neill D, et al. (2014). "Simultaneous rapid sequencing of multiple RNA virus genomes." Journal of virological methods 201: 68-72.
For Research Use Only.
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