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DIA Quantitative Proteomics

DIA Quantitative Proteomics

DIA refers to the data-independent acquisition, which is a new mass spectrometry data acquisition method developed in recent years. All parent ions within a specific mass range can be fragmented, and fragment ion information of all parent ions can be collected for protein qualitative and quantitative. Because DIA data acquisition mode divides the whole scanning range of mass spectrometry into several window areas, and selects, shatters and detects all ions in each window area in high speed and cycle, to obtain all the fragments information of all ions in the sample without omission or difference. Currently, popular proteomics research methods, such as iTRAQ, label-free and SILAC, all of them use DDA. Compared with DDA, DIA has better accuracy and repeatability, which is an important trend in proteomics research.

Schematic diagram of DIA quantitative technology Fig.1 Schematic diagram of DIA quantitative technology. (Ludwig C, et al. 2018.)

Application of DIA Quantitative Proteomics

Mass spectrometry analysis of protein enzymatic peptides by liquid chromatography-mass spectrometry (LC-MS-MS), combined with traditional DDA to construct a spectral library, the proteome can be identified and quantified to obtain information such as the source of peptides, proteins and their species, and the changes of protein expression.

Due to the panoramic data collection and quantification without labeling of protein quantitative DIA products, one of its main application areas is projects with large sample sizes (more than 10 comparison groups), and it has the advantages of fast, stable, and reproducible, so as to get rid of the constraints of labeling quantitative technology. It can be applied to precision medicine, cancer research, microbial environmental adaptability research and other fields.

Our Service Process

The service process of DIA quantitative proteomics - Creative Biogene

Service Offering

Creative Biogene’s DIA quantitative proteomics service provides results including,

  • Raw data
  • Experimental steps
  • Related mass spectrometry parameters
  • Details of identified relevant sites
  • Mass spectrum picture
  • Bioinformatics analysis report

Our bioinformatics analysis covers a wide range including data quality control, protein function annotation, protein quantitative analysis, protein difference analysis, enrichment analysis, differential protein interaction analysis and transcriptome correlation analysis, etc.

Sample Type

Protein solutions, tissue samples, cells and body fluid samples.

Turnaround Time

In general, our turnaround time is 2-5 weeks depending on the size of your project.

Advantages of Our Service 

  • High sensitivity and non-discriminatory access to all peptides information, and will not cause the loss of low-abundance protein information.
  • The number of scanning points is uniform, and the quantitative accuracy is high.
  • With good repeatability, and the quantitative correlation between repeated samples can reach more than 0.99.

Creative Biogene has excellent microbiology experts, well-established proteome technology platforms, and professional bioinformatics analysis to provide you with a one-stop service in DIA quantitative proteomics. Our services include sample preparation, enzymatic hydrolysis, mass spectrometric detection, data retrieval and bioinformatics analysis.

If you are interested in our services, please contact us for more details.

Reference

  1. Ludwig C, et al. (2018). "Data‐independent acquisition‐based SWATH‐MS for quantitative proteomics: a tutorial." Molecular systems biology. 14.8: e8126.
For Research Use Only.

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