In nature, Rochalimaea appears to be dependent on arthropod and vertebrate hosts for survival, similar to Rickettaia. The bacteria generally inhabit the outside of the cells of the arthropod host. Notably, it can be cultured in host cell-free medium.
Creative Biogene offers customizable Rochalimaea strain culture services. This service can help you obtain Rochalimaea cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.
Figure 1. Rochalimaea quintana.(From en.wikipedia.org)
Rochalimaea quintana is a Brevibacterium with a cell size of 0.2-0.5×1.0-1.6 microns. It has a cell wall and plasma membrane composed of 3 lamellae. The bacterium has no flagella or capsule and is gram-negative. Split by horizontal dichotomy. The bacteria can be cultured on a blood agar-based medium containing 6% inactivated horse serum and 4% lysed horse erythrocytes. In addition, the bacterium can also be grown in a liquid medium in which calf fetal blood serum, rather than calf serum, is used instead of hemoglobin. Rochalimaea quintana growth requires aeration and increased partial pressure of CO2. Colonies of Rochalimaea quintana on agar surfaces were 65-200 microns in diameter. Its colonies are round, convex, translucent and mucoid. Chicken embryos and cell cultures are not very satisfactory for the growth of this bacterium. Tetracycline and chloramphenicol are highly inhibitory to growth. Penicillin is less effective, and streptomycin is relatively ineffective. Rochalimaea quintana cells can metabolize succinate and glutamine, but not glucose. The pre-in vitro culture test showed that the bacteria did not completely lose its activity when treated with moist heat at 60°C for 30 minutes or dry at 80°C for 20 minutes. It can survive for several months in dry lice droppings. Rochalimaea quintana does not infect guinea pigs and other general laboratory animals. Experimental infections have only occurred in rhesus monkeys and humans. Rochalimaea quintana is the causative agent of trench fever. Among them, human is the main host, which is mainly transmitted by the human louse (Pediculus humanus). The bacteria grow in the gut of the lice and are excreted along with the feces, which are a source of infection in humans, and enter the skin during itchiness. There is no indication that the infection can damage the lice.
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The culture of Rochalimaea requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.