The transcriptome is the integrated set of RNA encoded in an organism's genome. The metatranscriptome covers all the RNAs encoded by an organism in a complex sample. Microbial transcriptome and metatranscriptome information are significant for understanding host-pathogen immune interactions, predicting resistance to specific antibiotics, quantifying changes in gene expression, and tracking disease progression. The next-generation RNA sequencing of eukaryotes, bacteria, viruses, and other microbes has become a standard way of analyzing transcriptome and metatranscriptome information.
RNA-seq can be used for unbiased strand specific identification of both common and novel transcripts. The wide dynamic range allows you to reliably identify high and low expression in microbial RNA-seq experiments. Multiple samples can be processed at once with a simplified workflow suitable for automation.
Our bacterial RNA sequencing makes a use of stranded RNA libraries, can estimate the transcript expression more accurately compared with non-stranded RNA-seq, especially for antisense RNA and other overlapping genes. This sequencing method specifically aims at prokaryotes with reference genomes, and can provide customers with efficient and thoughtful solutions for transcriptome analysis and gene structure analysis, etc.
Eukaryotic microbial RNA sequencing is significant for revealing the regulation of gene expression in eukaryotic cells, exploring the interaction between the host and pathogen, and monitoring disease progression. We also provide microbial single-cell RNA-seq to meet your various demands in eukaryotic microbes.
Viral RNA sequencing refers to the sequencing of all the viral transcriptional information, which is time-saving and economical. Our viral RNA sequencing is committed to offering you solutions at viral pathogens identification, viral evolution research, and new vaccines design, etc.
The RNA-seq experiments of eukaryotic microbes, bacteria, viruses and other microbes can be annotated and quantified for comprehensive microbial transcripts. Cellular RNA is extracted and converted into cDNA for the preparation of the sequencing library. Then sequence reads are mapped back to the reference genome, providing qualitative information about features such as exon junctions and splicing sites, and quantitative transcriptional data that can be compared in many experimental groups.
Creative Biogene is a forward-looking research institution and a leading customer service provider in the field of microbial RNA sequencing. Our experienced scientists choose the most suitable sequencing strategy and data analysis pipeline according to your samples and research purposes. We use the advanced high-throughput sequencing platform to provide you with RNA sequencing services for bacteria, eukaryotic microbes and viruses.
If you are interested in our services, please contact us for more details.