Antimicrobial peptides (AMPs) consist of molecules that act on the defense systems of numerous organisms toward multiple pathogens such as bacteria, fungi, parasites and viruses. These compounds have become extremely significant due to the increasing resistance of microorganisms to common antibiotics. However, the low quantity of peptides obtained from direct purification is, to date, still a remarkable bottleneck for scientific and industrial research development. Therefore, Creative Biogene has developed the main heterologous systems currently used for AMP production, including bacteria and fungi, reaching greater functional peptide production. Large-scale production of AMPs can be obtained using biotechnological tools, and the products may be applied in the pharmaceutical industry.
The Escherichia coli expression system is the most common among all the methods of genetically engineered bacteria to produce antimicrobial peptides. The clear genetic background, mature expression technology and fast growth of bacteria make it widely used. At present, the method of using Escherichia coli to produce antimicrobial peptides has been very mature and has been widely used. It is an effective way for the large-scale production of antimicrobial peptides.
Pichia pastoris is the most widely used expression system among the yeasts. But there is no stable episomal plasmid when expressing foreign antimicrobial peptide genes, almost all expression vectors, such as pPICZ and pIPC3k, can be directly integrated into the yeast genome. The host strains used in the expression system to produce antimicrobial peptides are derived from the original wild-type strain NRRL-Y11430. A variety of antibacterial peptides have been expressed in yeast, and have considerable expression levels.
Bacillus subtilis, as a genetically engineered bacteria, provides a good way for the production of antimicrobial peptides. We have successfully utilized the vectors to realize the expression of the fusion antimicrobial peptides in Bacillus subtilis. It can be achieved by artificially synthesizing the target gene sequence of the antimicrobial peptide, linking it with the expression vector, constructing the recombinant expression plasmids, transferring them into Bacillus subtilis, and successfully expressing the antibacterial peptides.
Biotechnologies, such as the construction of constitutive gene expression cassettes, inducible gene expression systems, and specific protein targeting systems for lactic acid bacteria, have been mature. The modified lactic acid bacteria hardly secrete proteases, which indirectly reduces the risk of antimicrobial peptides being internally decomposed. Antibacterial peptides have been successfully expressed in lactic acid bacteria. The humanized antimicrobial peptide has also achieved expression in lactic acid bacteria. The lactic acid bacteria expression system, as a new type of expression system, has the potential to produce antimicrobial peptides. It can also be used as a way for the development and large-scale production of antimicrobial peptides.
Creative Biogene is the premier institution to provide antimicrobial peptide expression services. We are dedicated to offering complete solutions for gene expression to meet your specific project needs. Simply let us know the sequences you want to express. We will propose the best strategy for you.