Microbial Biofilm

Microbes form biofilms in order to adapt to the living environment during their growth. The biofilm is a complex microbial community, which refers to an organized bacteria population attached to the surface of living or inanimate objects and wrapped by bacterial extracellular macromolecules. The formation of biofilm is generally divided into four stages, namely initial adhesion, biofilm maturation, biofilm dispersal and cellular detachment. Biofilms exist in wounds and can be found in many other situations, such as stones at the bottom of streams, shower floors, sewers, etc. Dental plaque is also a common biofilm, which can lead to dental caries and gum disease.

Microbial biofilms are closely related to the formation of microbial resistance, gene transfer and persistent infections. At present, the research focus on biofilms has gone deep into the signal transmission between microbes, the transfer of pathogenic genes, and how to interfere with the formation of biofilms. In addition, biofilm technology has been applied in sewage treatment and environmental protection engineering, biomaterial engineering and food industry.

Stages in the biofilm formation processFig.1 Stages in the biofilm formation process. (Omar A, et al. 2017.)

Technical Process

  • The process of microbial biofilm high-throughput sequencing analysis.

The process of microbial biofilm high-throughput sequencing analysis - Creative Biogene

Sample Type and Detection Type

  • The sample type is 3-5g microbial biofilm extract.
  • The requirements of DNA sample: total DNA ≥ 500ng, DNA concentration ≥ 10ng/μL, OD260/280 = 1.8 - 2.0.
  • The detection types are bacteria, fungi, etc.

What Can We Offer?

  • Isolation, cultivation and identification of biofilm microbes.
  • Study the composition, abundance and community structure of biofilm microbes.
  • Explore the differences between biofilm microbes in different environments.
  • Other solutions customized according to your research project.

The specific service content includes the following aspects, microbial isolation & cultivation, microbial identification, microbial sequencing, microbial proteomics, microbial metabonomics, microbial genome editing and bioinformatics analysis.

Bioinformatics analysis

OTU clustering Taxonomic analysis Phylogenetic analysis Analysis based on OUT Gene function  analysis
OTU distribution
Rarefaction curve
Shannon index curve
Rank abundance curve
Diversity index
Community composition analysis
Community correlation analysis
Community difference analysis
  • NMDS analysis
  • PCA
  • PCoA
  • LEfSe differential analysis
  • Network diagram
UniFrac analysis
Phylogenetic tree
OTU correlation analysis
  • Heatmap
  • Venn diagram
KEGG analysis
GO analysis
MetaCyc analysis
EggNOG analysis
CAZy analysis
CARD analysis

Our Advantages 

  • Our professional researchers have rich experience in the field of microbial biofilm.
  • We can combine the demands of customers, and negotiate to determine the content of solutions.
  • We have a well-established quality management system to ensure the quality of our services.

Microbial biofilm technology has been widely used and has become a research hotspot. Creative Biogene's skilled microbiologists have extensive experience of microbiological experiments and bioinformatics analysis. We will provide you with our optimal microbial biofilm solutions. With high quality and reasonable prices, we sincerely look forward to serving you.

If you are interested in our services, please contact us for more details.


  • Omar A, et al. (2017). "Microbial biofilms and chronic wounds." Microorganisms. 5.1: 9.

For research use only. Not intended for any clinical use.

For Research Use Only.
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