Bacillus Subtilis Genome Editing

Bacillus subtilis

Bacillus subtilis is a gram-positive bacterium, widely distributed in soil and decaying organic matter. Bacillus subtilis grows fast, and has a low nutritional requirement. It can efficiently secrete many proteins and metabolites, and does not produce toxins, and is a safe microbe without pathogenicity. Bacillus subtilis can produce active substances during the growth process, which has obvious inhibitory effects on pathogenic bacteria. In addition, it can synthesize many enzymes that are helpful for digestion. Bacillus subtilis can synthesize a variety of vitamins, improve the activity of interferons and macrophages in animals or humans, and can also be used as a microbial regulator in the industrial field to improve water quality and inhibit harmful microbes.

Application of Bacillus Subtilis Genome Editing

Bacillus subtilis is a model organism for studying the various facets of bacterial life and an important producer strain for industrial enzymes and valuable low-molecular-weight substances. It is considered a multifunctional microbe that has been used in many different fields. The gene editing of Bacillus subtilis helps to discover new gene functions, optimize metabolic pathways, increase the output of metabolites, realize industrial production, and help produce antibiotics and important industrial enzymes. It can also be used in the study of cellular functions and processes including protein secretion, sporulation, and signal transduction.

Application of Bacillus subtilis genome editing- Creative Biogene

Service Offering

Creative Biogene uses two methods to achieve Bacillus subtilis genome editing, they are,

  • Bacillus subtilis genome editing based on Rec
  • Bacillus subtilis genome editing based on CRISPR/Cas9

The specific content of the Bacillus subtilis genome editing service provided by Creative Biogene includes,

  • Bacillus subtilis gene knockout
  • Bacillus subtilis gene knock-in
  • Bacillus subtilis gene activation and inhibition
  • Bacillus subtilis gene point mutation

Turnaround Time

In general, our turnaround time is 4-8 weeks depending on the size of your project.

Advantages of Our Services 

  • Traceless gene editing technology, security is guaranteed.
  • The need for multiple gene knockouts can be realized simultaneously.
  • High gene editing efficiency and recombination efficiency.
  • We can combine the demands of customers, and negotiate to determine the content of services.
  • Short turnaround time and high cost performance.

Creative Biogene has excellent microbiology experts, well-established gene editing platforms, and professional bioinformatics analysis to provide you with a one-stop service in Bacillus subtilis genome editing. We believe that our services will make your project more effective.  

If you are interested in our services, please contact us for more details.


  1. Altenbuchner J. (2016). "Editing of the Bacillus subtilis genome by the CRISPR-Cas9 system." Applied and environmental microbiology. 82.17: 5421-5427.
  2. Wu G, et al. (2019). "Fast genome editing in Bacillus subtilis." Engineering in Life Sciences. 19.6: 471-477.
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