Water Microecology

Water Microecology

Water is an important environment for human survival. 71% of the earth’s surface is the ocean, which stores 97% of the earth’s water. The remaining 2% of the water is stored in glaciers and poles. Wherever there is water, there will be microbes. Water microbes mainly come from the soil, air, animal and plant residues, excrement, industrial production wastewater and municipal domestic sewage, etc.

According to the ecological characteristics of microbes in water bodies, they can be divided into two types. The first is the clean water aquatic microbes, which are mainly chemical autotrophic or photoautotrophic microbes, and they can grow in clean water that is not rich in organic substances, such as Thiobacillus, Cyanobacteria, Gallionella ferruginea, etc. The second is saprophytic aquatic microbes, which are mostly non-spore and gram-negative bacilli. Water also often becomes an important transmission route for pathogenic microbes of humans, animals and plants. Due to differences in human activities, temperature, oxygen content, and water fluidity, the types, quantities and distribution characteristics of microbes in various water bodies are quite different.

Technical Process

  • The process of water microbe high-throughput sequencing analysis.

The process of water microbe high-throughput sequencing analysis - Creative Biogene

  • The process of water microbe PCR-DEEG analysis.

The process of water microbe PCR-DEEG analysis - Creative Biogene

Sample Type and Detection Type

  • The sample types are DNA samples of water microbes from different sources (including rivers, lakes, glacial meltwater, seawater, sewage, etc.). Recommend to use our product - microbial sample collection.
  • The requirements of DNA sample: total DNA ≥ 500ng, DNA concentration ≥ 10ng/μL, OD260/280 = 1.8 - 2.0.
  • The detection types are bacteria, fungi, eukaryotic microbes, etc.

What Can We Offer?

Based on the 16S rRNA gene, 18S rRNA gene and ITS sequence, the next-generation sequencing technology is used to analyze the diversity of water microbes, and to explore the main factors affecting the microbial community and diversity in different water environments. There are a great number and varieties of microbes in water bodies. By analyzing the population structure and diversity of water bodies as well as studying their dynamic changes, it is possible to fully understand the composition of water microbes, to provide a reliable basis for optimizing community structure and regulating community functions.

The specific service content includes the following aspects, microbial isolation & cultivation, microbial identification, microbial sequencing, microbial proteomics, microbial metabonomics, microbial genome editing and bioinformatics analysis.

Bioinformatics analysis

OTU clustering Taxonomic analysis Phylogenetic analysis Analysis based on OUT Gene function  analysis
OTU distribution
Rarefaction curve
Shannon index curve
Rank abundance curve
Diversity index
Community composition analysis
Community correlation analysis
Community difference analysis
  • NMDS analysis
  • PCA
  • PCoA
  • LEfSe differential analysis
  • Network diagram
UniFrac analysis
PCoA
Phylogenetic tree
OTU correlation analysis
  • Heatmap
  • Venn diagram
PCA
RDA/CCA
KEGG analysis
GO analysis
MetaCyc analysis
EggNOG analysis
CAZy analysis
CARD analysis

Our Advantages 

  • We have experienced researchers and advanced bioinformatics platform.
  • We can customize the analysis items according to your needs.
  • Our comprehensive and detailed solutions provide you with a one-stop experience.

The entry of pathogenic microbes and parasites into water bodies will directly or indirectly endanger human health or affect fishery production. Therefore, the research on water microecology needs to be paid more attention. Creative Biogene is dedicated to providing customers with advanced microbial solutions, and the water microecology is one of our specialties. You can choose us without hesitation, we anticipate your presence.

If you are interested in our services, please contact us for more details.

For Research Use Only.
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