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De Novo Sequencing

De Novo Sequencing

De novo sequencing refers to sequencing a novel genome for the first time and requires the specialized assembly of sequencing reads. De novo sequencing can sequence the genome of a species without any reference genome information, and uses bioinformatics analysis methods to splice, assemble, and obtain the genome sequence map of the species, so as to promote the subsequent research of the species.

The process of de novo genome sequencing involves sequencing of small DNA fragments, then assembling the reads into longer sequences, and finally sorting the contigs to obtain the complete genome sequence. With the advent of low-cost, rapid next-generation sequencing technology, researchers can gain whole genome data of organisms that were previously considered to be too low priority for sequencing.

Application of De Novo Sequencing

Compared with other sequencing methods, de novo sequencing has the superiority of generating accurate reference sequences even for complex or polyploid genomes, elucidating highly similar or repetitive regions, as well as identifying structural variations and complex rearrangements.

De novo sequencing can be used used to obtain genomic information of bacteria or fungi, to explore genetic structure and function, to study the evolutionary origin of microbial populations, and to develop potential applications of these abundant microbes in medicine, disease, agriculture, and the environment. Specifically, the application of de novo sequencing including virulence research, drug resistance mechanism, molecular maker, evolution relationship and so on.

Application of de novo sequencing - Creative Biogene

Our Service Process

The service process of de novo sequencing - Creative Biogene

Service Offering

Each de novo sequencing project includes,

  • Collect and process samples, then extract the DNA of the sample.
  • Preliminary quality control inspection on all samples, and the QC report returned to review.
  • Using our optimized approach to prepare the library.
  • Whole genome sequencing at a depth depended on your research purpose.
  • Bioinformatics analysis report.

Our bioinformatics analysis covers a wide range including genomic component analysis, gene function analysis, comparative genomic analysis, etc.

Turnaround Time

In general, our turnaround time is 1-3 weeks depending on the size of your project.

Advantages of Our Services 

  • We have professional experts and we are in a leading position in microbial research.
  • Our team is very experienced and has completed numerous microbial de novo sequencing projects.
  • We have an advanced sequencing platform, allowing us to provide high-quality data, fast turnaround, and affordable price.
  • We develop personalized bioinformatics analysis solutions according to customer needs.

Creative Biogene has excellent microbiology experts, well-established high-throughput sequencing platforms, and professional bioinformatics analysis to provide you with a one-stop service in de novo sequencing. Our services include sample collection, quality control, library construction, sequencing and analysis.

If you are interested in our services, please contact us for more details.

Reference

  1.  Kisand V, et al. (2013). "Genome sequencing of bacteria: sequencing, de novo assembly and rapid analysis using open source tools." Bmc Genomics 14(1):211-211.
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