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Prosthecomicrobium Cultivation

Prosthecomicrobium Cultivation

Prosthecomicrobium is a unicellular Gram-negative bacterium with many protrusions extending from the cell in all directions. The protrusions are normally less than 2 microns in length and are tapered, tapering from the cell towards the blunt apex. Occasionally longer, non-tapering protrusions are produced. Cells divide by horizontal binary division. There are strains of Prosthecomicrobium that can exercise and some that cannot. Among them, the moving strain exhibited a tumbling circular motion.

Figure 1. Prosthecomicrobium.Figure 1. Prosthecomicrobium. (Oertli G.E., et al.; 2006)

Prosthecomicrobium is an organic chemotrophic bacteria. Its metabolism is aerobic, non-fermentative type. The bacteria are found in natural water.

Prosthecomicrobium Culture Service

Creative Biogene offers customizable Prosthecomicrobium strain culture services. This service can help you obtain Prosthecomicrobium cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Proathecomicrobium pneumaticum

Although the cells of Proathecomicrobium pneumaticum occasionally formed very long protrusions, the ratio of protrusions to cell diameter (approximately 1.0 μm) was all 1. In a clonal strain under certain growth conditions, some cells may appear without protrusions. Cells have air bubbles that are encapsulated. Colonies of Proathecomicrobium pneumaticum are unpigmented and non-motile. The bacteria can utilize ammonium but not nitrate as the sole nitrogen source for growth. Growth requires an organic carbon source and vitamins (biotin, thiamine and B12) and a temperature range of 9-42°C. Faster growth and higher yields were obtained above 30°C, but good growth at 30°C, indicating similar conditions to natural habitats. The optimum pH range is 6.0-6.5; in addition. It also grows well at pH 7.0.

Prosthecomicrobium enhydrum

Prosthecomicrobium enhydrum has a ratio of protrusion length to cell diameter (approximately 1.0 microns) of less than 0.5. The bacterium does not produce air bubbles, and has a characteristic tumbling motion with a subpolar to polar flagella. Provided that the growth medium contains appropriate vitamins and an organic carbon source, the bacteria can utilize ammonium instead of nitrate as the sole nitrogen source. Thiamine is required in the growth medium. Its colonies can be white, yellow or red. Growth temperature range is 9-37°C. Growth was faster and higher yields were obtained above 30°C, but growth was best at 30°C, indicating that this was similar to natural habitat conditions. The optimum growth pH is 7.0.

Microbial GMP Production

Creative Biogene's fermentation platform has Good Manufacturing Practices (GMP) and can provide customers with a wide range of high-quality microbial fermentation products such as active pharmaceutical ingredients, enzymes and various fine chemicals. In addition, our microbiology experts have completed the transformation and innovation of traditional processes through continuous breakthroughs in key technologies of microbial fermentation processes, and fully contributed to the smooth delivery of the project.

Production Capacity

Creative Biogene builds a world-class microbial fermentation technology platform, providing a variety of services from strain screening and optimization to fermentation production and product purification. We have many years of rich experience and provide good technical support for microbial GMP production.

Facility Display

As a leader in microbial production, Creative Biogene has comprehensive production process technology and high-volume manufacturing capabilities. Our goal is to help our customers develop streamlined and controlled manufacturing processes and to support customers throughout the entire product development process, from the R&D stage to market launch.

Device Example:

  • Fermentation, centrifugation and filtration upstream process equipment;
  • Fully automatic fermenters ranging in volume from 4,000L to 12,000L with a total capacity of over 100,000 liters;
  • From industrial-scale chromatography systems, membrane systems to larger-scale continuous centrifuges;
  • Recycling and Downstream Equipment;
  • Waste treatment equipment.

Why Choose Us?

The culture of Asticcacaulis requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.

References

  1. Bergey's Manual of Systematic Bacteriology Book Review Int. J. of Syst. Bact. 1985, p. 192.
  2. Oertli G.E., et al.; (2006) The Genera Prosthecomicrobium and Ancalomicrobium. In: Dworkin M., Falkow S., Rosenberg E., Schleifer KH., Stackebrandt E. (eds) The Prokaryotes. Springer, New York, NY. ISBN978-0-387-25495-1.
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