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Saccharomyces Cerevisiae Genome Editing

Saccharomyces cerevisiae

Saccharomyces cerevisiae is the most widely related yeast to humans. Besides its traditional use in baking and brewing, Saccharomyces cerevisiae is also an important host of industrial biotechnology. This yeast is the host of choice for biorefineries for ethanol fermentation, which uses food biomass or lignocellulosic feedstocks. Saccharomyces cerevisiae is also applied for producing a variety of enzymes and pharmaceutical proteins, such as insulin, hepatitis and human papillomavirus vaccines, and it is being developed to produce advanced biofuels as well as fine chemicals.

Saccharomyces cerevisiae has long been the most tractable organism in eukaryotic cell biology due to its genetic plasticity. Homologous recombination (HR) was superior to non-homologous end joining (NHEJ) in double strand break (DSB) repair, which greatly promoted the growth of Saccharomyces cerevisiae. For years, biologists have taken advantage of this preference, allowing for the site-specific installation of genetic material and genome editing with the precision of specific base pairs.

Application of Saccharomyces Cerevisiae Genome Editing

The CRISPR-Cas9 technology and homologous recombination technology are used to edit and modify the genome of Saccharomyces cerevisiae, so as to obtain yeast with better performance, thereby improving the application value of Saccharomyces cerevisiae in biopharmaceuticals, pollution control, energy and chemical bio-manufacturing industries.

Service Offering

Creative Biogene uses two methods to achieve Saccharomyces cerevisiae genome editing, they are,

  • Saccharomyces cerevisiae genome editing based on Rec
  • Saccharomyces cerevisiae genome editing based on CRISPR/Cas9

The specific content of the Saccharomyces cerevisiae genome editing service provided by Creative Biogene includes,

  • Saccharomyces cerevisiae gene knockout
  • Saccharomyces cerevisiae gene knock-in
  • Saccharomyces cerevisiae gene activation and inhibition
  • Saccharomyces cerevisiae gene point mutation

Turnaround Time

In general, our turnaround time is 4-8 weeks depending on the size of your project.

Advantages of Our Services 

  • Traceless gene editing technology, security is guaranteed.
  • The need for multiple gene knockouts can be realized simultaneously.
  • High gene editing efficiency and recombination efficiency.
  • We can combine the demands of customers, and negotiate to determine the content of services.
  • Short turnaround time and high cost performance.

Creative Biogene has excellent microbiology experts, well-established gene editing platforms, and professional bioinformatics analysis to provide you with a one-stop service in Saccharomyces cerevisiae genome editing. We believe that our services will make your project more effective.  

If you are interested in our services, please contact us for more details.

Reference

  1. Stovicek V, et al. (2015). "CRISPR–Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains." Metabolic Engineering Communications. 2: 13-22.
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