Protein identification uses mass spectrometry technology to identify all or specific proteins in cells, tissues, body fluids, as well as protein gels/liquids after separation, purification, and enrichment. A full understanding of protein types in a specific environment is the basis for proteomics research, and provides intuitive protein identification results for studying the occurrence and development of diseases and the growth and development of animals and plants. Protein identification and analysis products can be divided into the following three products according to the research object and content, protein full spectrum analysis, gel spot/strip identification and protein molecular weight determination.
Protein full spectrum analysis is an overall analysis method of the proteome, which can obtain as much protein information as possible through mass spectrometry. It is mainly used for a comprehensive analysis of the types of proteins contained in unknown samples. The protein full spectrum analysis based on mass spectrometry technology can provide reference information for high-throughput quantitative and modification analysis of proteins. Besides, the combination of full-spectrum analysis data and transcriptome data can complement each other and jointly verify gene annotation and functional information.
Gel spot/strip identification methods can be applied to pure or single protein samples. Use two-dimensional electrophoresis to obtain protein profiles, use relevant software to analyze the protein spots of interest, and use mass spectrometry to identify them to obtain detailed protein information. In addition, designated bands or less component protein fluids isolated from SDS-PAGE gel can also be identified by mass spectrometry.
Molecular weight is the basic physical and chemical property parameter of organic compounds. Whether the molecular weight is correct or not often represents the correctness of the structure of the measured organic compound and biological macromolecule. Molecular weight is also one of the most important parameters in the identification of polypeptide and protein. The accurate molecular weight of the protein is obtained by mass spectrometry, and the molecular weight of a single or multi-component protein can be detected.
Creative Biogene’s protein identification service provides results including,
Our bioinformatics analysis covers a wide range including data quality control, protein identification results, protein GO analysis, protein pathway analysis, relative protein abundance quantification of a single sample, and other customize analysis services.
In general, our turnaround time is 2-5 weeks depending on the size of your project.
Creative Biogene has excellent microbiology experts, well-established proteome technology platforms, and professional bioinformatics analysis to provide you with a one-stop service in protein identification. Our services include protein extraction, enzymolysis, HPLC, mass spectrometry and bioinformatics analysis, etc.
If you are interested in our services, please contact us for more details.