Thermoplasma cells are polymorphic, varying in shape from spherical (0.3–2μm) to filamentous structures. It reproduces by budding. Its cells lack a true cell wall, but are surrounded by a single three-layer membrane, about 10-12nm thick. The genus is not motile. Colonies on solid medium containing 1.2% ionic agar, up to about 0.3 mm in diameter; these colonies are somewhat flat, coarsely granular, and dark brown. Some of them exhibit a typical "fried egg" appearance with a zona pellucida around the perimeter. Grow in liquid base containing 0.02% (NH4)2SO4, 0.05% MgSO4, 0.025% CaCl2·H2O, 0.3% KH2PO4, 0.1% yeast extract and 1.0% glucose. At concentrations below 0.2%, cell harvest was proportional to the concentration of yeast extract; no growth was observed on the ether extract fraction of yeast. During the cultivation process, sterols are not required. Thermoplasma are strictly aerobic bacteria. The optimum temperature for its growth is about 59°C. Grow well between pH 0.96 and 3.0; pH optimum is about 1-2. Thermoplasma is a true saprophytic bacteria that has been isolated from a pile of burned cinders and acidic hot springs.
Creative Biogene offers customizable Thermoplasma strain culture services. This service can help you obtain Thermoplasma cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.
Figure 1. Thermoplasma acidophilum. (From microbewiki.kenyon.edu)
Thermoplasma acidophilam has the same characteristics as its genus.
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The culture of Thermoplasma requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.