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Pelonema Cultivation

Pelonema Cultivation

The cells of Pelonema are colorless and have a thin, smooth wall. Cells usually contain gas vacuole. The multicellular filaments of Pelonema are unbranched, straight or curved, and uniform in thickness. The filaments may or may not be constricted at the transverse septum. Filaments occasionally showed slow sliding movements. The bacterium reproduces by breaking its filaments, and it is unknown whether it has dormant cells.

Pelonema type.Figure 1. Pelonema type. (Alison E. Clark, et al.; 1978)

Pelonema Culture Service

Creative Biogene offers customizable Pelonema strain culture services. This service can help you obtain Pelonema cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Pelonema tenue 

The filaments of Pelonema tenue do not move, but occasionally do slow sliding movements. The shape of the filaments is straight or slightly curved, slightly constricted at the diaphragm. Protoplasts are highly refractive. Its cells have gas vacuole. Under natural conditions, it is common in deep freshwater and alkaline water, on the bottom of swamps, and on sludge in lakes.

Pelonema aphane 

The filaments of Pelonema aphane do not move, or move very slowly. The shape of the filaments is until slightly curved, slightly constricted at the diaphragm, and uniform in diameter. The cells are colorless and have a central gas vacuole. Under natural conditions, it is commonly found on the bottom of swamps and on the sludge of deep freshwater lakes. Also seen in the upper layers during the infancy cycle.

Pelonema pseudovacuolatum

The filaments of Pelonema pseudovacuolatum are immobile, uniform in diameter, straight to slightly curved, or irregularly coiled. Constricted very thinly at the transverse septum. The cells are cylindrical or slightly curved barrel-shaped, colorless, and have central gas vacuole. Under natural conditions, it is common in deep freshwater pools, lake bottom sludge, and occasionally among plankton.

Pelonema subtilissimum

The filaments of Pelonema subtilissimum do not move, the diameter is uniform, the shape is straight, and the diaphragm is not constricted. Its cells are colorless and have granular protoplasts and a central gas vacuole. Under natural conditions, it is commonly found in the stagnation layer of Blankvatn (an ancient lake site in Oslo, Norway).

Pelonema hyalinum

The filaments of Pelonema hyalinum do not move, or move only very slowly, are uniform in diameter, do not constrict at the diaphragm, and have transparent cells with a central gas vacuole. Found on the sludge bottoms of deep lakes.

Why Choose Us?

The culture of Pelonema requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.

References

  1. Bergey's Manual of Systematic Bacteriology Book Review Int. J. of Syst. Bact. 1985, p. 148.
  2. Alison E. Clark, et al.; The Occurrence of Gas-Vacuolate Bacteria in Lakes. Archives of Microbiology. 1978, volume 118, pages223–228.
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