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Staphylococcus Aureus Genome Editing

Staphylococcus Aureus Genome Editing

Staphylococcus aureus

Staphylococcus aureus is a representative of gram-positive bacteria and widely exists in the natural environment. Staphylococcus aureus is a common food-borne pathogenic microbe. Under appropriate conditions, it can produce enterotoxins and cause food poisoning. It is associated with high morbidity and mortality due to its obvious pathogenicity and multidrug resistance. In many countries, the emergence of antibiotic-resistant strains, such as methicillin-resistant and vancomycin-resistant Staphylococcus aureus, has rendered the bacteria as the cause of the infectious diseases with the highest mortality rate, emphasizing a dire need for novel drug-target exploration and drug development.

Creative Biogene has developed a fast and accurate CRISPR/cas9-mediated genome editing technology for Staphylococcus aureus. This technology combines the use of recombination to achieve efficient genome editing of Staphylococcus aureus, including gene deletion, single-base substitution mutation and gene insertion, making it possible to scalably and inexpensively engineer desired changes.

Application of Staphylococcus Aureus Genome Editing

Staphylococcus aureus genome editing will contribute to the search for solving various problems related to the function of specific genes and specific mutations. The development of new genome editing and screening tools will greatly facilitate the acquisition of unexplored drug targets, which will enable the effective discovery and verification of unidentified genes and pathways that lead to bacterial toxicity and drug resistance.

Service Offering

Creative Biogene uses two methods to achieve Staphylococcus aureus genome editing, they are,

  • Staphylococcus aureus genome editing based on Rec
  • Staphylococcus aureus genome editing based on CRISPR/Cas9

The specific content of the Staphylococcus aureus genome editing service provided by Creative Biogene includes,

  • Staphylococcus aureus gene knockout
  • Staphylococcus aureus gene knock-in
  • Staphylococcus aureus gene activation and inhibition
  • Staphylococcus aureus gene point mutation

Turnaround Time

In general, our turnaround time is 4-8 weeks depending on the size of your project.

Advantages of Our Services 

  • Traceless gene editing technology, security is guaranteed.
  • The need for multiple gene knockouts can be realized simultaneously.
  • High gene editing efficiency and recombination efficiency.
  • We can combine the demands of customers, and negotiate to determine the content of services.
  • Short turnaround time and high cost performance.

Creative Biogene has excellent microbiology experts, well-established gene editing platforms, and professional bioinformatics analysis to provide you with a one-stop service in Staphylococcus aureus genome editing. We believe that our services will make your project more effective.  

If you are interested in our services, please contact us for more details.

References

  1. Ran F, et al. (2015). "In vivo genome editing using Staphylococcus aureus Cas9." Nature. 520.7546: 186-191.
  2. Penewit K, et al. (2018). "Efficient and scalable precision genome editing in Staphylococcus aureus through conditional recombineering and CRISPR/Cas9-mediated counterselection." MBio. 9.1.
For Research Use Only.

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