Nonmetric multidimensional scaling (NMDS) analysis is a data analysis method that reduces research objects in multidimensional space to low-dimensional space for positioning, analysis and classification, while retaining the original relationship among objects. This method is no longer based on the distance matrix value, but based on the ranking order. It is a nonlinear model that can better reflect the ecological data. The primary feature of NMDS analysis is to treat the similarity or dissimilarity data between objects as a monotonic function of the distance between points. On the basis of maintaining the original data order relationship, replace the original data with new data columns of the same order for metric multidimensional scale analysis.
NMDS analysis is often used to compare the differences between sample groups. It can be based on the evolutionary relationship or quantitative distance matrix. The main difference between NMDS analysis and PCA analysis lies in the consideration of evolutionary information. In the NMDS plot, the points with different colors or shapes represent sample groups under different environments or conditions, the distance between the points represents the degree of difference, and the horizontal and vertical coordinates represent the relative distance.
Fig.1 Nonmetric multidimensional scaling (NMDS) analysis plot of T-RFLP profiles of bacterial 16S rRNA amplicons digested with the restriction enzyme Hae III. (Ito, M, et al. 2019)
Our services in terms of NMDS analysis include,
Analytical tools used in our NMDS analysis service include Qiime software, R language tool and other related tools. Qiime is used to calculate the beta diversity distance matrix, and the R language is used for NMDS analysis and mapping.
In general, our turnaround time is 2-4 weeks depending on the size of your project.
When there are too many samples or species, the NMDS analysis will be more accurate. Creative Biogene's NMDS analysis service is excellent in speed, accuracy and sensitivity. You can choose us with confidence, and we will provide you with our optimal service.
If you are interested in our services, please contact us for more details.