Microbial production of metabolites is a cost-effective and sustainable approach to producing high-value compounds. Microorganisms are relatively easy to grow and manipulate, and they have the potential to produce a wide range of metabolites with diverse chemical structures. There are several types of microbial metabolites that can be produced, including antibiotics, enzymes, organic acids, vitamins, and biofuels. These microbial natural products have revolutionized bioprocess technologies, agricultural sustainability, and biopharmaceuticals.
Table 1 Microbial Primary and Secondary Metabolites
The process of microbial production of metabolites typically involves selecting a suitable microorganism, optimizing growth conditions, and then inducing the production of the desired metabolite. This can be done by manipulating environmental factors such as temperature, pH, and nutrient availability. Genetic engineering techniques can also be used to modify the microorganism's metabolic pathways to enhance the production of specific metabolites.
Through fermentation, microorganisms growing on inexpensive carbon sources can produce valuable products such as amino acids, nucleotides, organic acids, and vitamins. Microorganisms have the potential to provide many petroleum-derived products as well as the ethanol necessary for liquid fuel. The role of primary metabolites and the microbes which produce them will certainly increase in importance.
Figure 1 Microbial primary metabolism
Microbial secondary metabolites are low molecular mass products, not essential for growth of the producing cultures. They include antibiotics, antitumor agents, cholesterol-lowering drugs, and others. They have unusual structures and are usually formed during the late growth phase of the producing microorganisms. Its synthesis can be influenced greatly by manipulating the type and concentration of the nutrients formulating the culture media. Our knowledge and manipulation have been useful either for setting fermentation conditions or for strain improvement.
Figure 2 Classification of reported Bacillus secondary metabolites. (I. Horak, et al. 2019)
Fermentation and Transformation
|Semi Pilot & Pilot Plants
|2-5 L fermenters, incubation chambers, master cell bank, etc.
|Cutting edge technology focused on versatility for all types of development projects (up to 2,000 L fermenter).
|Different fermentation chamber capacities.
Auxiliary tanks and continuous sterilizers.
Custom options for Microbial End-products
Creative Biogene offers CDMO services for various microbial biologics. We have the commercial experience and technological flexibility to scale manufacturing on a need-based or rolling basis. Our sites are growing globally as the expansion of facilities has increased the capacity for microbial systems. In addition, we have experience in developing processes for mRNA vaccines, monoclonal antibody manufacturing, pDNA, and Viral Vector (Lenti viral and Adeno Associated Virus) platforms.
If you are interested in our services, please contact us for more details.