consultation 

Root Microecology

Root Microecology

Root microbes

Root microbes refer to microbes that grow and reproduce within a few millimeters of soil directly affected by plant roots. There are bacteria, actinomycetes, fungi, algae and protozoa. They interact with plant roots and plants to form a mutualistic relationship. Root microbes are mainly bacteria, and Gram-negative bacteria are dominant.

Most root microbes are harmless to plants and have a promoting effect on plant growth. The metabolites produced during the proliferation of root microbes increase the absorption of phosphorus and other mineral elements by plants. In addition, the growth-stimulating hormones they secrete can also promote plant growth. Plants also secrete substances that inhibit the growth of microbes. Some root microbes secrete antibiotics, which can inhibit the reproduction of plant pathogens.

Technical Process

  • The process of root microbe high-throughput sequencing analysis.

The process of root microbe high-throughput sequencing analysis - Creative Biogene

  • The process of root microbe PCR-DEEG analysis.

The process of root microbe PCR-DEEG analysis - Creative Biogene

Sample Type and Detection Type

  • The requirements of DNA sample: total DNA ≥ 300ng, DNA concentration ≥ 10ng/μL, OD260/280 = 1.8 - 2.0.
  • The main detection types are bacteria and fungi, etc.

What Can We Offer?

We use high-throughput sequencing, PCR-DGGE, real-time fluorescent quantitative PCR and other methods to sequence DNA in samples. Through bioinformatics analysis, reveal the types of root microbes and their relative abundance and evolutionary relationship, explore microbial diversity, and study the correlation between root microbes and the environment.

The specific service content includes the following aspects, microbial isolation & cultivation, microbial identification, microbial sequencing, microbial proteomics, microbial metabonomics and bioinformatics analysis.

Bioinformatics analysis

OTU clustering Taxonomic analysis Phylogenetic analysis Analysis based on OUT Gene function  analysis
OTU distribution
Rarefaction curve
Shannon index curve
Rank abundance curve
Diversity index
Community composition analysis
Community correlation analysis
Community difference analysis
  • NMDS analysis
  • PCA
  • PCoA
  • LEfSe differential analysis
  • Network diagram
UniFrac analysis
PCoA
Phylogenetic tree
OTU correlation analysis
  • Heatmap
  • Venn diagram
PCA
RDA/CCA
KEGG analysis
GO analysis
MetaCyc analysis
EggNOG analysis
CAZy analysis
CARD analysis

Our Advantages 

  • We have experienced researchers and professional bioinformatics analysis.
  • We provide fast turnaround, high quality analysis solutions at competitive prices to our global customers.
  • We provide an interpretation of the final report and other personalized pre-sales and after-sales services.

Root microbes are mostly beneficial microbes, which can be used to repair organic pollutants. Therefore, the study of root microecology is very important for environmental protection and agricultural development. Creative Biogene is a forward-looking research institution in the field of root microecology. Our advanced analysis platforms are established to provide you with results of good reproducibility. More discounts, more surprises, we look forward to your selection.

If you are interested in our services, please contact us for more details.

For Research Use Only.

Have a question? Get a Free Consultation