Bacillus Subtilis Cultivation

Bacillus subtilis is an aerobic, Gram-positive soil bacterium. It secretes numerous enzymes to degrade a variety of substrates, enabling the bacterium to survive in a continuously changing environment. B. subtilis expression systems with good genetic stability are available for various purposes. It has excellent protein secretion ability and no strong codon preference, making it important host for the production of heterologous proteins. Moreover, the CRISPR/cas9 tool has been widely used in the genetic engineering of B. subtilis for production of industrial chemicals or enzymes, medicine and biomaterials. B. subtilis is generally recognized as safe (GRAS).

Applications of B. subtilis Cultivation Figure 1 Applications of B. subtilis Cultivation

Culture Media and Conditions

Luria–Bertani (LB) agar plate and LB medium has been used for the activation of glycerol stock strain and the cultivation of seed culture. M9 medium is used for growth curve determination and ribonucleic acid production.

Bacillus subtilis Cultivation Strategies

B. subtilis has excellent physiological characteristics and highly adaptable metabolism, which makes it easy to cultivate on cheap substrates. It grows fast and the fermentation cycle is usually around 48 h.

Adaptive laboratory evolution (ALE) is an important strategy to improve the fitness of microorganisms through selected environmental conditions and was widely used in metabolic engineering. Combining ALE and rational gene knockout provids potential strategies for improving B. subtilis cell growth.

Bacillus subtilis Fermentation Strategies

The application of fast-growing B. subtilis cell for different products may be different.Increased specific growth rate of B. subtilis may shortened the fermentation period, therefore, the productivity can be potentially improved for non-growth-coupled products. The utilization efficiency of production equipment can also be improved, which can reduce the cost of bioproduction.

Two-stage solid-state fermentation strategy is exploited and systematically optimized to enhance Bacillus subtilis growth and sporulation for increasing effective cell number in B. subtilis microbial ecological agents. The first stage focused on improving cell growth followed by the second stage aiming to enhance both cell growth and sporulation.

Our Facilities

We possess a mature fermentation platform consisting of large fermentation tanks, bioreactors, and large-scale purification systems, which enables us to provide high-purity and kilogram level products.

Bacillus Subtilis Cultivation

Creative Biogene has established a mature technical platform to culture various microorganisms that can be isolated from readily available sources. We have developed efficient strategies for microbial cultivation and fermentation. If you are interested in our Bacillus subtilis cultivation services, please contact us for more details.


  1. Su, Y., Liu, C., Fang, H. et al. Bacillus subtilis: a universal cell factory for industry, agriculture, biomaterials and medicine. Microb Cell Fact 19, 173 (2020).
  2. Yanfeng Liu, et al. Developing rapid growing Bacillus subtilis for improved biochemical and recombinant protein production. Metabolic Engineering Communications. 2020.
  3. Zhi-Min Zhao, et al. Enhancement of Bacillus subtilis Growth and Sporulation by Two-Stage Solid-State Fermentation Strategy. Processes. 2019, 7(10), 644.
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