Bacterial RNA Sequencing

Bacteria belong to prokaryotes, and prokaryotes lack membrane-bound nuclei and other organelles, meaning transcription, translation, and mRNA degradation can all occur at the same time. Prokaryotic transcription usually involves more than one gene and produces polycistron mRNAs that specify more than one protein, which is a major difference compared to eukaryotic transcripts.

Bacterial RNA Sequencing uses next-generation sequencing to reveal the presence and quantity of RNA at a specific time by analyzing the changes in cell transcriptome. Our bacterial RNA sequencing makes a use of stranded RNA libraries, can estimate the transcript expression more accurately compared with non-stranded RNA-seq, especially for antisense RNA and other overlapping genes. This sequencing method specifically aims at prokaryotes with reference genomes, and can provide customers with efficient and thoughtful solutions for transcriptome analysis and gene structure analysis, etc.

Application of Bacterial RNA Sequencing

Application of bacterial RNA sequencing- Creative Biogene

Our Service Process

The service process of bacterial RNA sequencing - Creative Biogene

Sample Requirements

Sample Type Library Type Amount Purity
Total RNA Bacterial RNA library ≥3μg OD260/280 = 1.8-2.2
OD260/230 ≥ 1.8

Service Offering

Each bacterial RNA sequencing project includes,

  • Collect and process samples, then extract the RNA of the sample.
  • Preliminary quality control inspection on all samples, and the QC report returned to review.
  • Using our optimized approach to prepare the library.
  • RNA sequencing at a depth and a length depended on your research purpose.
  • Bioinformatics analysis report.

Our bioinformatics analysis covers a wide range including novel transcript prediction, gene expression quantification, functional analysis, operon analysis, SNP and InDel, URT analysis and antisense transcript prediction, etc.

Turnaround Time

In general, our turnaround time is 1-2 weeks depending on the size of your project.

Advantages of Our Services 

  • We have professional experts and we are in a leading position in microbial research.
  • We constantly strive for excellence in quality to provide first-class services.
  • Our team is very experienced and has completed numerous microbial bacterial RNA sequencing projects.
  • Our advanced sequencing platform, allowing us to provide high-quality data and affordable price.
  • We can combine the needs of customers, negotiate to determine the content of services

Creative Biogene has excellent microbiology experts, well-established high-throughput sequencing platforms, and professional bioinformatics analysis to provide you with a one-stop service in bacterial RNA sequencing. Our services include sample collection, quality control, library construction, sequencing and analysis.

If you are interested in our services, please contact us for more details.


  1. Croucher J, et al. (2010). "Studying bacterial transcriptomes using RNA-seq." Current Opinion in Microbiology 13(5):619-624.
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