Bacteria belong to prokaryotes, and prokaryotes lack membrane-bound nuclei and other organelles, meaning transcription, translation, and mRNA degradation can all occur at the same time. Prokaryotic transcription usually involves more than one gene and produces polycistron mRNAs that specify more than one protein, which is a major difference compared to eukaryotic transcripts.
Bacterial RNA Sequencing uses next-generation sequencing to reveal the presence and quantity of RNA at a specific time by analyzing the changes in cell transcriptome. Our bacterial RNA sequencing makes a use of stranded RNA libraries, can estimate the transcript expression more accurately compared with non-stranded RNA-seq, especially for antisense RNA and other overlapping genes. This sequencing method specifically aims at prokaryotes with reference genomes, and can provide customers with efficient and thoughtful solutions for transcriptome analysis and gene structure analysis, etc.
|Sample Type||Library Type||Amount||Purity|
|Total RNA||Bacterial RNA library||≥3μg||OD260/280 = 1.8-2.2
OD260/230 ≥ 1.8
Each bacterial RNA sequencing project includes,
Our bioinformatics analysis covers a wide range including novel transcript prediction, gene expression quantification, functional analysis, operon analysis, SNP and InDel, URT analysis and antisense transcript prediction, etc.
In general, our turnaround time is 1-2 weeks depending on the size of your project.
Creative Biogene has excellent microbiology experts, well-established high-throughput sequencing platforms, and professional bioinformatics analysis to provide you with a one-stop service in bacterial RNA sequencing. Our services include sample collection, quality control, library construction, sequencing and analysis.
If you are interested in our services, please contact us for more details.