Thioploca consists of flexible filaments composed of many sheets, usually sulfur-containing particles, in parallel or braided bundles surrounded by a common sheath. The width of its sheath is not fixed. The number of filaments in each sheath varies. The diameters of the filaments in a sheath may be exactly the same, or they may be quite different. The sheath is covered in debris. Each individual filament slides independently, and the last segment of the filament is often pointed.
Figure 1. Thioploca. (From wikipedia)
Thioploca exhibits typical behavior of microaerophilic microorganisms. Pure cultivation of Thioploca has so far been unsuccessful. In anoxic seawater with added nitrates, natural populations can survive in situ temperatures around 13°C for months, but they require a delicate balance of sulfide, nitrate and oxygen concentrations, making enrichment more challenging.
Creative Biogene offers customizable Thioploca strain culture services. This service can help you obtain Thioploca cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.
According to the NCBI database, a total of four Thioploca have been effectively published: two freshwater species (Thioploca ingrica and Thioploca schmidlei) and two marine species (Thioploca araucae and Thioploca chileae).
Thioploca schmidlei has been identified from various regions in Central Europe and is found in freshwater sludge.
Thioploca ingrica was identified from isolates from all over Central Europe and from lakes in Ireland and was found in brackish and freshwater sludge.
Thioploca minima was identified throughout Central Europe and in Irish lakes, and found in freshwater sludge.
Thioploca mixta was identified in Lake Costanci. There is a similar combination of filaments of 1 µm and 2.0-4.5 µm in diameter in Irish lakes, making this species a dubious taxonomic entity, found in freshwater sludge.
The culture of Thioploca requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.