The cells of Nevskia are rod-shaped, 0.7-2.0x2.4-12μm in size, often slightly curved. In some cases, the cells are enveloped with mucus, while in most cases a transparent stalk is formed on one side of the cell perpendicular to the long axis of the bacteria. As a result of the division of mature cells, the stalk bifurcates and leads to the formation of floating colonies with a size of up to 80 microns. They can be spherical, branched or annular and often form more or less dense membranes. No metal casing on handle.
Nevskia's extracellular mucus was dissolved in 1% KOH. The mucus was stained with very dilute methyl violet, but the cells were not. Cells are usually filled with colorless globules with strong reflections. These spheres are not sulfur spheres or poly-beta hydroxy titanate spheres, they can be dyed with Burdon's grease dye and are soluble in 70% alcohol. Young cultures have 1 to 3 polar flagella and are mobile.
Gram stain for Nevskia was negative. It is aerobic bacteria. Originally isolated from aquarium water.
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Figure 1. Photomicrograph of a formaldehyde-fixed and toluidine blue-stained enrichment of N. ramosa. (Glöckner FO, et al.;1998)
Nevskia ramose is the type species of Nevskia, and its morphology is consistent with the description of the same genus.
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The culture of Nevskia requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.