The shape of the vegetative cells of Nannocystis is a short column with a pure round or spherical end. The sporangia are solitary.
The vegetative cells of Nannocystis exedens are short and thick rods with blunt ends, 1.4-2.5×3.5 microns in size. With its growth, the bacteria vegetative cells can gradually become oval, cubic or spherical.
The sporangia were oval or spherical, with great variation in shape, ranging in size from 3.5-6×40-110 microns. Among them, there are more smaller sporangia, which are embedded in the matrix.
Figure 1. Nannocystis exedens. (From BacDive)
Myxospores differ little from vegetative cells, are oval or spherical, and have dense optical properties.
The vegetative colonies of Nannocystis exedens are light orange to reddish-purple and have a unique way of eroding agar, and have been cultivated on agar agar with vitamin B12-supplemented yeast extract Ca2+. It can also be grown on Casitone medium, but high concentrations will inhibit growth. Nannocystis exedens can produce catalase that can hydrolyze gelatin and casein. The bacteria almost completely dissolve yeast and Micrococcus luteus. Nannocystis exedens does not hydrolyze starch and cellulose during culture. Its optimum temperature is 30°-36°C. Can be isolated from different soils in Samoa, USA and Germany.
The culture of Nannocystis requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.