Lactobacillus Genome Editing


Lactobacillus is a type of sporeless, gram-positive bacteria whose main product of fermented sugar is lactic acid. It is a kind of probiotics that can ferment carbohydrates into lactic acid, hence the name, Lactobacillus. Lactobacillus is widely distributed in meat, milk, vegetables and other food and its products. In addition, it is also widely present in the intestines of livestock and poultry and a few clinical samples. Among them, Lactobacillus in the oral and intestinal environments of humans and other mammals are important members of the normal microbial flora in a specific area.

A large number of research data show that Lactobacillus can promote the growth of the animal, regulate the normal flora of the gastrointestinal tract, maintain the microecological balance, thereby improving gastrointestinal function, increasing food digestibility as well as biological potency, reducing serum cholesterol, controlling endotoxin, inhibiting the growth of spoilage bacteria in the intestines, and improving the body's immunity.

Application of Lactobacillus Genome Editing

Lactobacillus plays an irreplaceable role in promoting human health. Applications of Lactobacillus gene editing include editing native genes to alter the immune regulatory properties of probiotics, and acting as antimicrobial production factories to alter the microbiota, and developing new antimicrobial agents for the production of a variety of proteins and metabolites. Besides, the genome editing technology can enhance the native antibacterial activity and increase the yield of antibacterial agents.

Application of Lactobacillus genome editing- Creative Biogene

Service Offering

Creative Biogene uses two methods to achieve Lactobacillus genome editing, they are,

  • Lactobacillus genome editing based on Rec
  • Lactobacillus genome editing based on CRISPR/Cas9

The specific content of the Lactobacillus genome editing service provided by Creative Biogene includes,

  • Lactobacillus gene knockout
  • Lactobacillus gene knock-in
  • Lactobacillus gene activation and inhibition
  • Lactobacillus gene point mutation

Turnaround Time

In general, our turnaround time is 4-8 weeks depending on the size of your project.

Advantages of Our Services 

  • Traceless gene editing technology, security is guaranteed.
  • The need for multiple gene knockouts can be realized simultaneously.
  • High gene editing efficiency and recombination efficiency.
  • We can combine the demands of customers, and negotiate to determine the content of services.
  • Short turnaround time and high cost performance.

Creative Biogene has excellent microbiology experts, well-established gene editing platforms, and professional bioinformatics analysis to provide you with a one-stop service in Lactobacillus genome editing. We believe that our services will make your project more effective.  

If you are interested in our services, please contact us for more details.


  1. Pijkeren J, et al. (2018).  "Genome editing of food-grade Lactobacilli to develop therapeutic probiotics." Bugs as drugs: therapeutic microbes for the prevention and treatment of disease. 5: 389-408.
  2. Leenay R, et al. (2019). "Genome editing with CRISPR‐Cas9 in Lactobacillus plantarum revealed that editing outcomes can vary across strains and between methods." Biotechnology journal. 14.3: 1700583.
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