CARD Analysis

With the discovery and use of antibiotics, more and more resistant strains have emerged. Drug resistance will increase the difficulty and cost of disease treatment. The identification and mining of resistance genes can help us uncover the resistance mechanism and provide references for disease treatment and drug development. The research on resistance genes has attracted extensive attention.

CARD (the Comprehensive Antibiotic Research Database) is a resistance gene database released by Canadian biotech personnel in 2013. It contains all the resistance information in the ARDB database. The core of the CARD database is ARO (Antibiotic Resistance Ontology), which is a term constructed by the database to associate antibiotic modules and their targets, resistance mechanisms, gene mutations and other information. At present, CARD annotation is widely used for microbial gene annotation analysis, especially in the prediction of metagenomic sequencing results.

Distribution of ARG types (in relation to antibiotic types) detected in the 13 samples Fig.1 Distribution of ARG types (in relation to antibiotic types) detected in the 13 samples. (Wang J, et al. 2018)

Service Offering

The resistance gene prediction analysis can be achieved by choosing BLAST and RGI (Resistance Gene Identifier) modes,

  • BLAST relies on the BLAST software in NCBI to align the sequence with the CARD reference sequence to obtain relevant annotation information.
  • RGI is the software developed by the CARD database team to predict the sequence of resistance genes based on proteins, that is, to predict the sequence of resistance genes through protein homology and protein variation.

Turnaround Time

In general, our turnaround time is 2-4 weeks depending on the size of your project.

Advantages of Our Services 

  • Our professional researchers have rich experience in CARD analysis.
  • We are going to combine the demands of customers, and negotiate to determine the content of services.
  • Our service has fast turnaround time, high quality and reasonable price.
  • We regularly accept audits from our customers to make sure that we meet their high standards.

The application of antibiotics has an important impact on the human and the natural environment. Studies on the resistance genes in water, feces, soil, active silt and human intestinal environment using metagenomics have been reported in the literature. Creative Biogene uses CARD to provide a complete resistance gene analysis program, which can deeply analyze the development trend of resistance genes and discuss the impact of antibiotics on the natural environment and human health. Combining rich project experience, strict data quality control and professional analysis process, we will ensure that the project is carried out accurately and quickly. We anticipate your presence.

If you are interested in our services, please contact us for more details.


  1. Wang J, et al. (2018). "Metagenomic analysis of antibiotic resistance genes in coastal industrial mariculture systems." Bioresource technology. 253: 235-243.
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