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Saccharomyces cerevisiae Cultivation

Saccharomyces cerevisiae Cultivation

Yeast is the earliest microorganism that was domesticated and utilized by humans. The Caucasians learned to use yeast to brew alcoholic beverages in 8000 BC; in 1845, Louis Pasteur discovered and isolated pure yeast;christian Hansen, a contemporary of Pasteur, began to study yeast in depth in order to brew high-quality beer, and pure cultured and classified yeast study research.

Saccharomyces cerevisiae is the earliest microorganism to achieve industrial-scale manufacturing, and it is also the most widely used strain among yeast species. Its industrial application process also reflects the development of the biological industry. Saccharomyces cerevisiae, also known as baker's yeast or budding yeast. Saccharomyces cerevisiae is the most widely related yeast to humans, not only because it is traditionally used to make food such as bread and steamed bread and wine, but also used as a eukaryotic model organism in modern molecular and cellular biology, and its role is equivalent to model organism Escherichia coli. Saccharomyces cerevisiae is the most commonly used biological species in fermentation. Saccharomyces cerevisiae cells are spherical or oval, 5–10μm in diameter. The method of reproduction is budding.

Yeast cells have two life forms, haploid and diploid. Haploids have a simpler life cycle and reproduce by mitosis. It usually dies in times of high environmental stress. Diploid cells (the predominant form of yeast) also reproduce by simple mitosis, but can enter meiosis when external conditions are poor, producing a series of haploid spores. Haploids can mate and re-form diploids.

All strains of S. cerevisiae can be grown under aerobic conditions using glucose, maltose and trehalose. Galactose and fructose have been shown to be the two fermenting sugars that work best when Saccharomyces cerevisiae can be cultivated. The ability of yeast to use different sugars depends on whether they grow aerobically or anaerobic. Some strains cannot grow anaerobic on sucrose and trehalose.

All strains can use ammonia and urea as nitrogen sources for the medium, but cannot use nitrate because they lack the ability to reduce it to ammonium ions. In addition, S. cerevisiae can also use most amino acids, small peptides and nitrogen bases as nitrogen sources. However, histidine, glycine, cystine and lysine are not available. Saccharomyces cerevisiae does not secrete proteases, so extracellular proteins cannot be metabolized.

Saccharomyces cerevisiae cultures also require phosphorus and sulfur assimilation. Good growth of yeast also requires some metals, such as magnesium, iron, calcium and zinc.

Saccharomyces cerevisiae Culture Service

Creative Biogene offers customizable Saccharomyces cerevisiae strain culture services. This service can help you obtain Saccharomyces cerevisiae cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Figure 1. Flow chart of yeast biomass manufacturing. (ZHANG Yao, et al.; 2022)Figure 1. Flow chart of yeast biomass manufacturing. (ZHANG Yao, et al.; 2022)

Microbial GMP Production

Creative Biogene's fermentation platform has Good Manufacturing Practices (GMP) and can provide customers with a wide range of high-quality microbial fermentation products such as active pharmaceutical ingredients, enzymes and various fine chemicals. In addition, our microbiology experts have completed the transformation and innovation of traditional processes through continuous breakthroughs in key technologies of microbial fermentation processes, and fully contributed to the smooth delivery of the project.

Production Capacity

Creative Biogene builds a world-class microbial fermentation technology platform, providing a variety of services from strain screening and optimization to fermentation production and product purification. We have many years of rich experience and provide good technical support for microbial GMP production.

Facility Display

As a leader in microbial production, Creative Biogene has comprehensive production process technology and high-volume manufacturing capabilities. Our goal is to help our customers develop streamlined and controlled manufacturing processes and to support customers throughout the entire product development process, from the R&D stage to market launch.

Device Example

  • Fermentation, centrifugation and filtration upstream process equipment;
  • Fully automatic fermenters ranging in volume from 4,000L to 12,000L with a total capacity of over 100,000 liters;
  • From industrial-scale chromatography systems, membrane systems to larger-scale continuous centrifuges;
  • Recycling and Downstream Equipment;
  • Waste treatment equipment.

Our Training Technology

  • High-density culture technology
  • Equipment integration technology

Why Choose Us?

The culture of Saccharomyces cerevisiae requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.

References

  1. Li M J, Borodina I. Application of synthetic biology for production of chemicals in yeast Saccharomyces cerevisiae. FEMS Yeast Research. 2015,15( 1):1-12.
  2. Di Serio M, et al.; Quantitative analysis of the key factors affecting yeast growth. Industrial & Engineering Chemistry Research. 2003, 42(21):5109-5116.
  3. Hong H S, et al.; Simulation of gas-inducing reactor couples gas-liquid mass transfer and biochemical reaction. Biochemical Engineering Journal. 2014, 91: 1-9.
  4. ZHANG Yao, et al.; The Industrial Applications of Saccharomyces cerevisiae. China Biotechnology. 2022, 42(1 /2):26-36.
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