Kusnezovia Cultivation

The cells of Kusnezovia are spherical, 0.5-1.5μm in diameter, and are connected by filaments approximately 0.1-0.2μm in diameter. The bacteria do not move, grow attached, and reproduce by budding. After removing the coated manganese shell with oxalic acid, it can be stained with carbolic acid gentian violet. In addition, microcolonies and arrays of spherical cells can be observed by underwater peloscopes. They are attached to the preexposed capillary wall with a handle and hang freely down into the capillary. Microcolony shapes are extremely diverse, the following types of manganese-encapsulated microcolonies have been observed: arrow-like shoots, open lily-of-the-valley leaves, wine glasses with serrated edges or compound grape-like branches resembling lichens structure. In peloscopes, the formations may also be similar to sticks or large branched candlesticks, always pointing downwards towards the reducing formation with their wider parts. Colonies can form layers in dark brown formations. Budding can be observed in the accumulation monolayer spherical cells of filamentous definitive populations or leaf-like structures. Kusnezovia is a distinct microaerobic bacteria. During the cultivation process, the addition of decomposed fungal hyphae can promote its development. During growth, the bacteria can oxidize and precipitate manganese and very little iron. Kusnezovia was originally discovered in some mud samples from Lake Ukshezero, Karelian SSR.

Kusnezovia Culture Service

Creative Biogene offers customizable Kusnezovia strain culture services. This service can help you obtain Kusnezovia cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Figure 1. iron bacteria.( O. V. Kutovaya, et al.; 2012)

Kusnezovia polymorpha

The morphology of Kusnezovia polymorpha is the same as the described genus.

Microbial GMP Production

Creative Biogene's fermentation platform has Good Manufacturing Practices (GMP) and can provide customers with a wide range of high-quality microbial fermentation products such as active pharmaceutical ingredients, enzymes and various fine chemicals. In addition, our microbiology experts have completed the transformation and innovation of traditional processes through continuous breakthroughs in key technologies of microbial fermentation processes, and fully contributed to the smooth delivery of the project.

Production Capacity

Creative Biogene builds a world-class microbial fermentation technology platform, providing a variety of services from strain screening and optimization to fermentation production and product purification. We have many years of rich experience and provide good technical support for microbial GMP production.

Facility Display

As a leader in microbial production, Creative Biogene has comprehensive production process technology and high-volume manufacturing capabilities. Our goal is to help our customers develop streamlined and controlled manufacturing processes and to support customers throughout the entire product development process, from the R&D stage to market launch.

Device Example:

• Fermentation, centrifugation and filtration upstream process equipment;
• Fully automatic fermenters ranging in volume from 4,000L to 12,000L with a total capacity of over 100,000 liters;
• From industrial-scale chromatography systems, membrane systems to larger-scale continuous centrifuges;
• Recycling and Downstream Equipment;
• Waste treatment equipment.

Why Choose Us?

The culture of Kusnezovia requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.