The cells of Kusnezovia are spherical, 0.5-1.5μm in diameter, and are connected by filaments approximately 0.1-0.2μm in diameter. The bacteria do not move, grow attached, and reproduce by budding. After removing the coated manganese shell with oxalic acid, it can be stained with carbolic acid gentian violet. In addition, microcolonies and arrays of spherical cells can be observed by underwater peloscopes. They are attached to the preexposed capillary wall with a handle and hang freely down into the capillary. Microcolony shapes are extremely diverse, the following types of manganese-encapsulated microcolonies have been observed: arrow-like shoots, open lily-of-the-valley leaves, wine glasses with serrated edges or compound grape-like branches resembling lichens structure. In peloscopes, the formations may also be similar to sticks or large branched candlesticks, always pointing downwards towards the reducing formation with their wider parts. Colonies can form layers in dark brown formations. Budding can be observed in the accumulation monolayer spherical cells of filamentous definitive populations or leaf-like structures. Kusnezovia is a distinct microaerobic bacteria. During the cultivation process, the addition of decomposed fungal hyphae can promote its development. During growth, the bacteria can oxidize and precipitate manganese and very little iron. Kusnezovia was originally discovered in some mud samples from Lake Ukshezero, Karelian SSR.
Creative Biogene offers customizable Kusnezovia strain culture services. This service can help you obtain Kusnezovia cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.
Figure 1. iron bacteria.( O. V. Kutovaya, et al.; 2012)
The morphology of Kusnezovia polymorpha is the same as the described genus.
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The culture of Kusnezovia requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.