Chloropseudomonas are rod-shaped bacteria that reproduce by binary division and move with polar flagella. Chloropseudomonas are Gram-negative bacteria. The cells contain bacteriochlorophyll c and carotenoids, and all the pigments are located in long oval sacs, which are below and attached to the cytoplasmic membrane. Bacterial cells do not contain air bubbles.
Chloropseudomonas are anaerobic bacteria. In the presence of hydrogen sulfide, photosynthesis can be carried out, and spherical elemental sulfur is produced as an intermediate oxidation product, which is precipitated in the extracellular medium. During photosynthesis, molecular hydrogen can be used as an electron donor and molecular oxygen is not produced. The cell suspension appears yellow-green in different shades due to photosynthetic pigments.
Chloropseudomonas ethylica has a cell size of 0.7-0.9 × 1.0-1.5 μm and moves with one polar and subpolar flagella. Single cells are colorless, and the color of cell suspensions is green.
Figure 1. Electron micrograph of Chloropseudomonas ethylica cells. (B. H. GRAY, et al.; 1973)
Chloropseudomonas ethylica is an obligate photoautotrophic and anaerobic bacterium. Optimum growth pH: 7.0-7.2, Optimum growth temperature: 25-30°C. If in the original habitat, higher concentrations of salts (NaCl, MgC12) may be required. The electron donors for photosynthesis are sulfides, sulfur, and organic carbon compounds. The electron donors for photosynthesis are sulfides, sulfur, and organic carbon compounds. Chloropseudomonas ethylica is photosynthesizable to ethanol, glucose, maltose and pyruvate in the presence of bicarbonate and trace amounts of sulfide. Certain strains also utilize thiosulfate, other sugars, acetate, formate, glycerol, lactate, mannitol and propanol.
The culture of Chloropseudomonas requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.