The rarefaction technique was developed by Howard Sanders in the biodiversity analysis of Marine benthic ecosystems in 1968. The rarefaction curve is to randomly select a certain number of individuals from the sample, and count the number of species represented by these individuals. The curve is composed of the number of individuals and species. It can be used to compare the abundance of species in samples with different sequencing data volumes, and explain whether the amount of sequencing data of a sample is reasonable.
By random sampling of sequences, the rarefaction curve is constructed with the number of sequences extracted and the number of OTUs they can represent. When the curve tends to be flat, it indicates that the sequencing data volume is reasonable. More data volume will only produce a few new OUT. On the other hand, it indicates that more new OTU may be generated by further sequencing. Therefore, the sequencing depth of the sample can be obtained by making the rarefaction curve.
Fig.1 Rarefaction curves for the 12 samples showing the diversity detected compared with the predicted total diversity. (Sauvain L, et al. 2014)
Rarefaction is not only a technique for measuring species diversity, but also for understanding diversity at a higher taxonomic level. It is most common to sample the number of species to predict the number of genera in a particular community. Rare techniques are used to quantify the species diversity of newly studied ecosystems, including the human microbiome, as well as applied research in community ecology, such as understanding the impact of pollution on communities and other management applications.
The services we provide in terms of rarefaction curves include,
Analytical tools used in our rarefaction curve service include mothur software, Perl language tool and other related tools. Mothur is used to analyze the rarefaction, and the perl language tool is used to make the curve.
In general, our turnaround time is 2-4 weeks depending on the size of your project.
Creative Biogene is a forward-looking research institution and a leading customer service provider in the field of rarefaction curve. We use advanced analysis platforms to provide you with results of good reproducibility. The service we offer is excellent in speed, accuracy and sensitivity. You can choose us with confidence.
If you are interested in our services, please contact us for more details.