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Intestinal Microecology

Intestinal Microecology

Intestinal microbes

After human beings are born, due to the exposure of the surrounding environment and diet, a stable microecology of intestinal flora is gradually established. Therefore, there is a complex microecosystem composed of about 1x1014 microbes in the human intestine, the number of which is more than 10 times that of the body's cells, including more than 1,000 kinds of anaerobic bacteria and aerobic bacteria.

The intestinal microecosystem is the largest and most important microecosystem in the human body. These intestinal microbes and their metabolites play an extremely significant role in the metabolism of nutrients, the body's development, immunity and diseases. Many studies have shown that intestinal microbes are directly related to the pathogenesis of many diseases, such as cancer, obesity, and neurodegenerative diseases.

Research Direction

The main research directions of intestinal microbes including,

  • Look for the difference in intestinal flora between the disease group and the healthy group.
  • Study the intestinal flora of the drug or diet intervention group.
  • Intestinal flora of the same population but at different growth and development stages.
  • Study the intestinal flora of a population in the development process of a certain disease.
  • Study the microbes in multiple body parts of the same population.

Technical Process

  • The process of intestinal microbe high-throughput sequencing analysis.

The process of intestinal microbe high-throughput sequencing analysis - Creative Biogene

  • The process of intestinal microbe PCR-DEEG analysis. DEGG (Denatured gradient gel electrophoresis) has become one of the main molecular biology methods to study the structure of the microbial community.

The process of intestinal microbe PCR-DEEG analysis - Creative Biogene

Sample Type and Detection Type

  • The sample types are feces or intestinal contents of humans, mice, monkeys, sheep, fish, and other animals. Recommend to use our product - microbial sample collection.
  • The requirements of DNA sample: total DNA ≥ 200ng, DNA concentration ≥ 10ng/μL, OD260/280 = 1.8 - 2.0, ensure that DNA is not degraded, and repeated freezing-thawing should be avoided during sample preservation.
  • The detection types are bacteria, fungi, actinomycetes, etc.

What Can We Offer?

  • Cultivate, isolate and identify the strains of intestinal microbes.
  • Study the function of intestinal microbes.
  • Gene editing of intestinal microbes.
  • Reveal the types, relative abundance and evolutionary relationship of intestinal microbes.
  • Explore the diversity of intestinal microbes and study their relationship with the environment.

The specific service content includes the following aspects, microbial isolation & cultivation, microbial identification, microbial sequencing, microbial proteomics, microbial metabonomics, microbial genome editing and bioinformatics analysis.

Bioinformatics analysis

OTU clustering Taxonomic analysis Phylogenetic analysis Analysis based on OUT Gene function  analysis
OTU distribution
Rarefaction curve
Shannon index curve
Rank abundance curve
Diversity index
Community composition analysis
Community correlation analysis
Community difference analysis
  • NMDS analysis
  • PCA
  • PCoA
  • LEfSe differential analysis
  • Network diagram
UniFrac analysis
PCoA
Phylogenetic tree
OTU correlation analysis
  • Heatmap
  • Venn diagram
PCA
RDA/CCA
KEGG analysis
GO analysis
MetaCyc analysis
EggNOG analysis
CAZy analysis
CARD analysis

Our Advantages 

  • Our services cover almost all aspects of microbiology, allowing you to enjoy a one-stop experience.
  • We can customize the service and combine it with quick turnaround at an affordable cost.
  • Simplified workflows with comprehensive bioinformatics analysis and high quality publishable data delivery.
  • Provide interpretation of the final report and other personalized pre-sales and after-sales services.

Creative Biogene has outstanding experience in microbiological research design and execution. The intestinal microecology is closely related to human health and has received extensive attention. We provide a wide range of services in the field of intestinal microecology, with excellent performance in speed, accuracy and sensitivity, and we deliver results of good quality and reproducibility. We look forward to your selection.

If you are interested in our services, please contact us for more details.

Reference

  1. Lin S, et al. (2019). "Role of intestinal microecology in the regulation of energy metabolism by dietary polyphenols and their metabolites." Food & nutrition research. 63.
For Research Use Only.

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