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Achromatium Cultivation

Achromatium Cultivation

Achromatium cells are round, oval or cylindrical. Its terminal cells are semi-circular and 5-33x15-125 microns in size. It divides by constriction in the middle of the cell. Motile strains of the bacterium move in a slow-rotating, quiver-like motion that depends on what is present within the cell. There are no photosynthetic pigments in the bacterial cells. In natural habitats, cells may contain sulfur droplets and large calcium carbonate spheres. The bacterium has not been cultivated in pure culture, and its dormant period is unknown.

Morphology of Achromatium cells.Figure 1. Morphology of Achromatium cells. (Sina Schorn, et al.; 2019)

Achromatium is a micro-aerobic bacteria. During the cultivation process, sulfide needs to be added.

Achromatium Culture Service

Creative Biogene offers customizable Achromatium strain culture services. This service can help you obtain Achromatium cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Achromatium oxaliferum 

The cells of Achromatium oxaliferum are round, oval or round simple, the shortest axis is 5 microns and the longest axis is 100 microns. It generally contains small sulfur particles and large calcium carbonate crystals with high refractive index. The latter may disappear under suitable circumstances. Cells can be isolated by treating them with acetic acid. The calcium carbonate-containing cells are particularly heavy, so these bacteria are commonly found on the bottoms of rivers and ponds, usually on or in sludge. Gently shake the muddy water in the beaker to concentrate them easily. Gram staining of Achromatium oxaliferum was negative, as was the contact enzyme. The species described are mainly from freshwater and saltwater sludges, but are also found in seawater sludges.

Achromatium volutans

The cells of Achromatium volutans range from spherical cells about 5 microns in diameter to oval cells as long as 40 microns. Gram stain reaction unknown. Generally,it contains sulfur particles, but no calcium carbonate precipitates in the cells. It is found in seawater sludge containing H2S and in decomposed seagrass.

Why Choose Us?

The culture of Achromatium requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.

References

  1. Bergey's Manual of Systematic Bacteriology Book Review Int. J. of Syst. Bact. 1985, p. 147.
  2. Sina Schorn, et al.; Cell Architecture of the Giant Sulfur Bacterium Achromatium oxaliferum: Extra-cytoplasmic Localization of Calcium Carbonate Bodies. FEMS Microbiology Ecology. 2019, 96(2)
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