Cupriavidus necator is a Gram-negative soil bacterium belonging to the class Proteobacteria. Its colony size is 1-2mm. Cupriavidus necator colonies are circular with a raised center and neat edges. The colony is opaque light yellow in color, its surface is smooth and bright, and its texture is viscous.
Figure 1. Metalophil production strain Cupriavidus necator with PHA granules (bright intracellular inclusions) imaged by STEM. (Martin Koller, et al., 2012)
Cupriavidus necator can be cultured on a nutrient broth medium (NA/NB) containing 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, 20.0g of agar (without liquid medium), and 1.0L of distilled water. Its optimum growth pH is 7.0.
Cupriavidus necator is a hydrogen oxidizing bacterium capable of growing at the interface of anaerobic and aerobic environments. It can be easily adapted to both heterotrophic and autotrophic lifestyles. Both organic compounds and hydrogen can be used as energy sources. In addition, Cupriavidus necator can also perform aerobic or anaerobic respiration by denitrifying nitrate and/or nitrite to nitrogen gas. When grown under autotrophic conditions, Cupriavidus necator fixes carbon through the reducing pentose phosphate pathway. The optimum growth temperature is 30°C. More interestingly, Cupriavidus necator produces polyhydroxyalkanoate (PHA) plastic when exposed to excess sugar substrate. PHA can accumulate to around 90% of the dry cell weight. Therefore, it can be used for PHA synthesis.
Figure 2. STEM picture of Cupriavidus necator cells.(Martin Koller, et al., 2012)
Creative Biogene offers customizable Cupriavidus necator strain culture services. This service can help you obtain Cupriavidus necator cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.
Creative Biogene's fermentation platform has Good Manufacturing Practices (GMP) and can provide customers with a wide range of high-quality microbial fermentation products such as active pharmaceutical ingredients, enzymes and various fine chemicals. In addition, our microbiology experts have completed the transformation and innovation of traditional processes through continuous breakthroughs in key technologies of microbial fermentation processes, and fully contributed to the smooth delivery of the project.
Creative Biogene builds a world-class microbial fermentation technology platform, providing a variety of services from strain screening and optimization to fermentation production and product purification. We have many years of rich experience and provide good technical support for microbial GMP production.
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The culture of Cupriavidus necator requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.