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Cupriavidus necator Cultivation

Cupriavidus necator Cultivation

Cupriavidus necator is a Gram-negative soil bacterium belonging to the class Proteobacteria. Its colony size is 1-2mm. Cupriavidus necator colonies are circular with a raised center and neat edges. The colony is opaque light yellow in color, its surface is smooth and bright, and its texture is viscous.

Figure 1. Metalophil production strain Cupriavidus necator DSM 545 with PHA granules (bright intracellular inclusions) imaged by STEM. (Martin Koller, et al., 2012)Figure 1. Metalophil production strain Cupriavidus necator DSM 545 with PHA granules (bright intracellular inclusions) imaged by STEM. (Martin Koller, et al., 2012)

Cupriavidus necator can be cultured on a nutrient broth medium (NA/NB) containing 3.0g of beef extract, 10.0g of peptone, 5.0g of NaCl, 20.0g of agar (without liquid medium), and 1.0L of distilled water. Its optimum growth pH is 7.0.

Cupriavidus necator is a hydrogen oxidizing bacterium capable of growing at the interface of anaerobic and aerobic environments. It can be easily adapted to both heterotrophic and autotrophic lifestyles. Both organic compounds and hydrogen can be used as energy sources. In addition, Cupriavidus necator can also perform aerobic or anaerobic respiration by denitrifying nitrate and/or nitrite to nitrogen gas. When grown under autotrophic conditions, Cupriavidus necator fixes carbon through the reducing pentose phosphate pathway. The optimum growth temperature is 30°C. More interestingly, Cupriavidus necator produces polyhydroxyalkanoate (PHA) plastic when exposed to excess sugar substrate. PHA can accumulate to around 90% of the dry cell weight. Therefore, it can be used for PHA synthesis.

Cupriavidus necator Culture Service

Figure 2. STEM picture of Cupriavidus necator cells.(Martin Koller, et al., 2012)Figure 2. STEM picture of Cupriavidus necator cells.(Martin Koller, et al., 2012)

Creative Biogene offers customizable Cupriavidus necator strain culture services. This service can help you obtain Cupriavidus necator cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.

Microbial GMP Production

Creative Biogene's fermentation platform has Good Manufacturing Practices (GMP) and can provide customers with a wide range of high-quality microbial fermentation products such as active pharmaceutical ingredients, enzymes and various fine chemicals. In addition, our microbiology experts have completed the transformation and innovation of traditional processes through continuous breakthroughs in key technologies of microbial fermentation processes, and fully contributed to the smooth delivery of the project.

Production Capacity

Creative Biogene builds a world-class microbial fermentation technology platform, providing a variety of services from strain screening and optimization to fermentation production and product purification. We have many years of rich experience and provide good technical support for microbial GMP production.

Facility Display

As a leader in microbial production, Creative Biogene has comprehensive production process technology and high-volume manufacturing capabilities. Our goal is to help our customers develop streamlined and controlled manufacturing processes and to support customers throughout the entire product development process, from the R&D stage to market launch.

Device Example:

  • Fermentation, centrifugation and filtration upstream process equipment;
  • Fully automatic fermenters ranging in volume from 4,000L to 12,000L with a total capacity of over 100,000 liters;
  • From industrial-scale chromatography systems, membrane systems to larger-scale continuous centrifuges;
  • Recycling and Downstream Equipment;
  • Waste treatment equipment.

Why Choose Us?

The culture of Cupriavidus necator requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.

References

  1. Yabuuchi, et al., Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol Immunol. 1995, 39 (11): 897–904.
  2. Davis, D.; et al., Proposal to reject the genus Hydrogenomonas: Taxonomic implications. Int J Syst Bacteriol. 1969, 19 (4): 375–390.
  3. Pohlmann, A.; et al., Genome sequence of the bioplastic-producing Knallgas bacterium Ralstonia eutropha H16. Nature Biotechnology. 2006, 24 (10): 1257–1262.
  4. Cramm, R.; et al., Genomic View of Energy Metabolism in Ralstonia eutropha H16. J Mol Microbiol Biotechnol. 2009, 16 (1–2): 38–52.
  5. Martin Koller, et al., Whey Lactose as a Raw Material for Microbial Production of Biodegradable Polyesters. Polyester. InTech. 2012, pp.51-92.
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