Rickettsia is a Brevibacterium with a cell size of 0.30.6×0.8-2.0 microns. Some species can be as long as 4 microns before cell division. The bacterial genus is both aflagellate and non-capsular, but an outer layer of amorphous material is occasionally seen in electron micrographs. Its Gram stain was negative. Rickettsia has never been cultured outside of host cells. Growth usually occurs within the protoplasm, but not in the vacuole, and sometimes in the nucleus of some vertebrate and arthropod cells. Its organism grows in endoderm cells along the yolk sac of the developing chicken embryo. In addition, this bacterial genus grows extremely well in vitro in chick embryo cells and in certain established mammalian cell lineages. It can produce patches on chick embryonic fibroblasts. The optimum growth temperature of this genus is 32°-35°C. Rickettsia reproduces exclusively by horizontal binary division. In chicken embryos, the division time is about 8 hours in the rapid growth phase. Para-phenyline inhibits the growth of Rickettsia, but this inhibition is relieved by para-hydroxybenzoic acid. Sulfonamides do not affect their growth. In addition, the following antibiotics inhibited its growth: Chloramphenicol, 0.08 mg; Chloramphenicol, 0.05 mg; Oxytetracycline, 0.008 mg; Erythromycin, 0.08 mg. Penicillin and streptomycin have little effect on the bacteria in eggs, but these antibiotics can have significant effects in cell culture. This bacterial genus is rapidly inactivated at 56°C and is generally unstable after isolation from host components. It can be preserved in diluents containing proteins, such as skim milk or plasma proteins, or in solutions consisting of sucrose, potassium phosphate and glutamate (SPG). Rickettsia is a human pathogen. Humans are the repositories of typical species and the occasional host of other species. Small rodents and other vertebrates store or transmit these bacteria. Arthropods play a major role in the life cycle of all species, are often the primary reservoir, and are almost the only intermediate natural spreaders among vertebrates.
Creative Biogene offers customizable Rickettsia strain culture services. This service can help you obtain Rickettsia cultures for subsequent scientific research. This service allows you to skip the complicated and tedious groping of culture conditions, which helps to speed up research on this strain.
Figure 1. Rickettsia rickettsia. (From en.wikipedia.org)
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The culture of Rickettsia requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.