Escherichia Coli Genome Editing

Escherichia coli

Escherichia coli was discovered by Escherichia in 1885, it accounts for 1% of the intestinal bacteria and it is a kind of gram-negative Brevibacterium with blunt ends and sporeless. Escherichia coli is a conditional pathogenic bacteria, which mainly resides in the intestines of humans or animals. A small number of Escherichia coli is toxic and can cause diseases. Most of the Escherichia coli distributed in nature is not pathogenic. Its metabolic activity can inhibit the growth of microbes that decompose protein in the intestine, reduce the harm of protein decomposition products to the human body, and can also synthesize vitamins. It will not cause disease under normal living conditions. If Escherichia coli is detected in water and food, it can be considered as an indicator of fecal contamination, because the number of coliforms (or coliform value) is often used as a hygienic standard for drinking water, food or medicine.

Application of Escherichia Coli Genome Editing

Escherichia coli is one of the most studied microbes due to its clear heritage background, simple technical operation and simple cultivation conditions. Genome editing of Escherichia coli can discover new special enzyme genes and functional genes related to important metabolic processes and metabolite production. It is widely used in production and the transformation of traditional industries and technology, which helps people to promote the rapid development of modern biotechnology.

Application of Escherichia coli genome editing- Creative Biogene

Service Offering

Creative Biogene uses two methods to achieve Escherichia coli genome editing, they are,

  • Escherichia coli genome editing based on Rec
  • Escherichia coli genome editing based on CRISPR/Cas9

The specific content of the Escherichia coli genome editing service provided by Creative Biogene includes,

  • Escherichia coli gene knockout
  • Escherichia coli gene knock-in
  • Escherichia coli gene activation and inhibition
  • Escherichia coli gene point mutation

Turnaround Time

In general, our turnaround time is 4-6 weeks depending on the size of your project.

Advantages of Our Services 

  • Traceless gene editing technology, security is guaranteed.
  • The need for multiple gene knockouts can be realized simultaneously.
  • High gene editing efficiency and recombination efficiency.
  • We can combine the demands of customers, and negotiate to determine the content of services.
  • Short turnaround time and high cost performance.

Creative Biogene has excellent microbiology experts, well-established gene editing platforms, and professional bioinformatics analysis to provide you with a one-stop service in Escherichia coli genome editing. We believe that our services will make your project more effective.  

If you are interested in our services, please contact us for more details.


  1. Jiang Y, et al. (2015). "Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system." Applied and environmental microbiology. 81.7: 2506-2514.
  2. Zhao D, et al. (2016). "Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9." Microbial cell factories. 15.1: 205.
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