In initial molecular phylogenetic studies of protists, the 18S rRNA gene was used as a source of the DNA markers. But mainly due to low phylogenetic resolutions at the genus and species levels, 18S rRNA has been replaced by 28S rRNA. However, the limited availability of 28S rRNA sequence data prevents the use of protein-coding genes in various molecular comparisons.
Instead, recent high-throughput DNA sequencing techniques have relied on partial 18S rRNA sequences as molecular DNA markers. The use of 18S rRNA is advantageous because there is a large amount of sequence data in public databases such as GenBank. Additionally, the 18S rRNA in eukaryotes contains eight regions (V1-V9, no V6 region), and there are both conserved regions and variable regions. The conserved regions reflect the genetic relationship between biological species, while the variable regions reflect the differences between species and are suitable for classification standards at the species level and above. Among them, the V4 region has the most complete database information, and the best classification effect, which is the best choice for 18S rRNA gene analysis and annotation.
At present, the 18SrRNA gene has been widely used in the identification and molecular systematics research of eukaryotic microbes such as microalgae and protozoa.
Fig.1 Secondary structure of the 18 S rRNA from the dinoflagellate, Karenia brevis. (Ki J. 2012)
|Service Package||Service Content||Period|
|Eukaryotic microbes 18S rRNA identification 1||
|Eukaryotic microbes 18S rRNA identification 2||
|Gene library construction of eukaryotic microbial 18S rDNA||
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