Eukaryotic Microbial 18S Sequencing

In initial molecular phylogenetic studies of protists, the 18S rRNA gene was used as a source of the DNA markers. But mainly due to low phylogenetic resolutions at the genus and species levels, 18S rRNA has been replaced by 28S rRNA. However, the limited availability of 28S rRNA sequence data prevents the use of protein-coding genes in various molecular comparisons.

Instead, recent high-throughput DNA sequencing techniques have relied on partial 18S rRNA sequences as molecular DNA markers. The use of 18S rRNA is advantageous because there is a large amount of sequence data in public databases such as GenBank. Additionally, the 18S rRNA in eukaryotes contains eight regions (V1-V9, no V6 region), and there are both conserved regions and variable regions. The conserved regions reflect the genetic relationship between biological species, while the variable regions reflect the differences between species and are suitable for classification standards at the species level and above. Among them, the V4 region has the most complete database information, and the best classification effect, which is the best choice for 18S rRNA gene analysis and annotation.

At present, the 18SrRNA gene has been widely used in the identification and molecular systematics research of eukaryotic microbes such as microalgae and protozoa.

Secondary structure of the 18 S rRNA from the dinoflagellate Fig.1 Secondary structure of the 18 S rRNA from the dinoflagellate, Karenia brevis. (Ki J. 2012)

Our Service Process

The service process of eukaryotic microbial 18S rRNA sequencing - Creative Biogene

Service Offering

Service Package Service Content Period
Eukaryotic microbes 18S rRNA identification 1
  • Sequencing of PCR products
  • Sequence amplification of 18S rDNA
  • Sequencing alignment analysis
1-2 weeks
Eukaryotic microbes 18S rRNA identification 2
  • Sequence amplification of 18S rDNA
  • Cloning and sequencing of related genes
  • Sequencing alignment analysis
1-2 weeks
Gene library construction of eukaryotic microbial 18S rDNA
  • Gene library construction of eukaryotic microbial 18S rDNA
  • Development of RFLP markers
  • Analysis of eukaryotic microbial community diversity
5-6 weeks

Creative Biogene's eukaryotic microbial 18S sequencing service provides results including,

  • Electrophoretogram of PCR products
  • Sequencing peak file and sequencing report
  • Sequence alignment and identification results
  • RFLP markers
  • Gene library of eukaryotic microbial 18S rDNA.
  • Eukaryotic microbes community diversity analysis

Advantages of Our Services

  • We will customize the experimental scheme according to your requirements.
  • We have experienced researchers and advanced sequencing platform.
  • Our services are undertaken to GMP standards.
  • We have a well-established quality management system and have passed ISO 9001:2015 certification.
  • We regularly accept audits from our customers to ensure that we meet their high standards.

Creative Biogene is an expert in microbial identification services and has accumulated years of experience in eukaryotic microbial 18S rRNA sequencing. Our advanced sequencing platform ensures that we provide you with efficient standard service procedures, high-quality experimental data and comprehensive bioinformatics analysis. Our services including all processes from DNA extraction of eukaryotic microbes to PCR amplification, purification, sequencing, and analysis.

If you are interested in our services, please contact us for more details.


  1. Ki J. (2012). “Hypervariable regions (V1-V9) of the dinoflagellate 18S rRNA using a large dataset for marker considerations.” Journal of Applied Phycology. 24(5):1035-1043.
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