Lamprocystis Cultivation

Lampocystis cells are spherical to oval, typically double spherical before cell division, and reproduce by binary division. The bacteria have polar hairs and move with polar hairs. Cells can be singly or in quadruplexes, aggregated into a considerable area, which is buried in mucus. Extended colonies can be branched or even reticulated. This aggregate breaks into smaller clusters and slightly rounded colonies, which are made motile by the flagella of the individual cells. From a taxonomic point of view, lamprocystis is a Gram-negative bacteria. The cells contain bacteriochlorophyll a and carotenoids, both of which are located on the cystic intima. There are air bubbles in the center of the cell.

Figure 1. The colonies of purple sulfur bacteria of Lamprocystis genus. (Kushkevych, I., et al.; 2021)

Lampocystis is an anaerobic bacteria. Photosynthesis is possible in the presence of hydrogen sulfide, when they produce and store spherical elemental sulfur as an intermediate oxide in the bubble-free part of the cell. Molecular hydrogen can act as an electron donor. During photosynthesis, molecular oxygen is not released. Due to photosynthetic pigments, the cell suspension showed different colors from purple to red.

Cultivation Services of Lamprocystis

Lamprocystis roseopersicina

The cell diameter of Lampocystis roseopersicina is 3.0-3.5 microns. Individual cells are grey, and cell suspensions are red, purplish red to violet. Lampocystis roseopersicina is an obligate photoautotroph and is strictly anaerobic. The optimum growth pH range is: 6.5-7.6; the optimum growth temperature: 20°-25°C. Photosynthetic electron donor: sulfide or sulfur. Acetate and pyruvic acid can be photoassimilated in the presence of sulfide and bicarbonate. Under natural conditions, Lamprocystis roseopersicina lives mostly in exposed hydrogen sulfide-containing mud and stagnant water or hot springs.

Why Choose Us?

The culture of Lamprocystis requires specific formulations of growth media for use in cloning, plasmid DNA preparation, and protein expression. Creative Biogene offers a selection of bacterial growth media and custom services for your specific application. If you are interested in our microbial anaerobic and aerobic culture platform, please contact us for more details.

References

1. Bergey's Manual of Systematic Bacteriology Book Review Int. J. of Syst. Bact.; July 1985, p. 42.
2. Kushkevych, I., et al.; Molecular Physiology of Anaerobic Phototrophic Purple and Green Sulfur Bacteria. International Journal of Molecular Sciences. 2021, 22(12), 6398.